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Muscular amino acid and nucleoside extract and pharmaceutical composition thereof

A carnosine glycoside and extract technology, applied in the field of medicine, can solve problems such as instability of hydrolytic enzymes, increased stability of insoluble particles, and destruction of active ingredients

Active Publication Date: 2013-07-10
HUNAN WUZHOUTONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art scheme represented by the above-mentioned technical scheme, in order to obtain qualified carnosine glycoside extract, a very high temperature and adding a hydrolase are used, and a higher temperature will destroy the active ingredients, and the process of adding a hydrolase It is more complex and costly, and the hydrolytic enzyme is also unstable and needs to be strictly controlled
The dosage form of carnosine glycosides is injection, and because active substances such as polypeptides and amino acids are prone to oxidation, condensation, polymerization and other changes during storage, it is easy to cause a significant increase in insoluble particles and poor stability

Method used

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  • Muscular amino acid and nucleoside extract and pharmaceutical composition thereof
  • Muscular amino acid and nucleoside extract and pharmaceutical composition thereof
  • Muscular amino acid and nucleoside extract and pharmaceutical composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Preparation of Carnosine Glycoside Extract

[0061] Take a total of 1000g of myocardium and muscle (weight ratio 1:1) of a healthy rabbit, remove the connective tissue, wash it with water for injection until it is bloodless, and grind it in a meat grinder. The weight ratio of the ground tissue and water for injection is 0.68 Mix at a ratio of :1, adjust the pH to 5.7 with acetic acid, homogenize with a high-speed tissue grinder, adjust the temperature of the homogenate to 24°C, and keep the temperature for 1 hour; freeze the homogenate at -30°C for 12 hours and then store it at 40°C Thaw and repeat this process 3 times; place the homogenate under an ultrasonic field with a power of 0.8KW and a frequency of 25KHz, add potassium chloride with a weight of 2.5% of the homogenate weight to the homogenate, keep the temperature at 20°C and start stirring. The stirring speed is 250 rev / min, and it is stirred under this ultrasonic field for 2 hours. After 2 hours, the ...

Embodiment 2

[0062] Example 2 Preparation of Carnosine Glycoside Extract

[0063] Take a total of 1000g of myocardium and muscle (weight ratio 1:1) of a healthy rabbit, remove the connective tissue, wash it with water for injection until it is bloodless, and grind it in a meat grinder. The weight ratio of the ground tissue and water for injection is 0.72 Mix at a ratio of :1, adjust the pH to 6.0 with acetic acid, homogenize with a high-speed tissue grinder, adjust the temperature of the homogenate to 27°C, and keep the temperature constant for 1 hour; freeze the homogenate at -32°C for 12 hours and then store it at 44°C Thaw and repeat this 3 times; place the homogenate under an ultrasonic field with a power of 0.8KW and a frequency of 25KHz, add potassium chloride with a weight of 2.5% of the homogenate weight to the homogenate, keep the temperature at 25°C and start stirring. Stirring speed is 250 rpm, stirring in this ultrasonic field for 2 hours, after 2 hours, place the homogenate in...

Embodiment 3

[0064] Example 3 Preparation of sarcosinoside pharmaceutical composition injection (5ml)

[0065] Take the carnosine glycoside extract prepared in Example 1, measure the content of polypeptide and hypoxanthine in the solution, adjust the concentration of the solution, add water for injection to 1000ml to make the concentration of polypeptide: 1.74mg / ml, and the concentration of hypoxanthine: 0.25mg / ml; add 1.5g of sorbitol, 0.2g of thioglycerol, and 1.0g of poloxamer 188 to the sarcosaminoglycan glycoside solution in turn, stir and dissolve at 32°C, filter with a 0.22μm filter membrane, subpackage after passing the test, and heat at 121°C Sterilize for 20 minutes to obtain the carnosine glycoside pharmaceutical composition injection.

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Abstract

The invention relates to a muscular amino acid and nucleoside extract containing polypeptide, amino acid, nucleoside, nucleotide and other medicinal components. The extract is prepared by crushing cardiac muscles and muscles of a rabbit of which the connective tissue is removed to be mixed with water, homogenizing, freeing and performing ultrasonic treatment; adding potassium chloride into the homogenate, and performing ultrasonic agitation; and centrifuging to obtain supernate, filtering with a filter membrane, and performing ultrafiltration. The invention also provides a muscular amino acid and nucleoside medicinal composition injection and lyophilized powder, wherein the muscular amino acid and nucleoside medicinal composition consists of the following components: 1000ml of muscular amino acid and nucleoside solution, 1.5g of sorbitol, 0.2g of thioglycerol, and 1.0g of poloxamer 188; and the concentration of the polypeptide in the muscular amino acid and nucleoside solution is between 1.58 and 1.92mg / ml, and the concentration of hypoxanthine is between 0.22 and 0.28mg / ml. The muscular amino acid and nucleoside pharmaceutical composition lyophilized powder is prepared by lyophilizing 1,000ml of muscular amino acid and nucleoside solution, 40g of dextran 40, 0.2g of thioglycerol, 1.0g of poloxamer 188, and 0.5g of hydroxypropyl betadex. The muscular amino acid and nucleoside extract and the pharmaceutical composition thereof have a good effect of improving the stability of muscular amino acid and nucleoside and maintaining low insoluble particles.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a carnosine glycoside extract and a pharmaceutical composition thereof. Background technique [0002] Carnosine glycosides are extracted from healthy rabbit muscles and myocardium, containing atrial peptides, calcitonin gene-related peptides, adenosine triphosphate (adenosine) and other nucleotides (nucleosides), more than 20 kinds of amino acids and their metabolic intermediates Drugs with active substances. It can promote the metabolism of the body. For muscle atrophy, nervous edema, cerebrovascular accidental paralysis, neurasthenic syndrome, etc. Ischemic heart, cerebrovascular disease, diabetes, microvascular disease. congestive heart failure. Various types of hypertension and its complications. Hemiplegia, cerebral arteriosclerosis, muscular atrophy, neurasthenia syndrome caused by cerebrovascular accident. Prevention and treatment of angina, myocardial infarction. To...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/34A61K47/34A61K9/08A61K9/19A61P9/04A61P9/10A61P9/12A61P21/00A61P7/10A61P25/00A61P3/10A61P9/02A61K47/10A61K47/40
Inventor 曾艺胡成忠
Owner HUNAN WUZHOUTONG PHARMA
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