Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment

A separation device and experimental technology, which is applied in the field of experimental biological separation devices, can solve the problems of being unable to meet large-scale and high-throughput operations, affecting the normal growth and reproduction of Daphnia magna, and not having a high degree of automation, so as to achieve easy implementation , ingenious design, convenient operation

Inactive Publication Date: 2013-07-24
CHANGZHOU ENVIRONMENTAL MONITORING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The deficiencies of the separation method of the above-mentioned national standard are: 1) in the standard method, the shelling produced by the reproduction of Daphnia magna and the algae pellets fed are not removed, and these substances will adhere to the body of Daphnia magna, causing the Movement is affected, which affects the normal growth and reproduction of Daphnia magna
2) The standard method adopts the nesting method to carry out the actual operation process, which is more cumbersome, and the screens of different sizes must be backwashed every time, and the separation speed is relatively slow
3) The automation degree of the standard method is not high enough to meet the large-scale and high-throughput operations in the future

Method used

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  • Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment
  • Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment

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Experimental program
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Embodiment 1

[0026] See figure 1 , the device for separating female and young daphnia used in daphnia experiment in this embodiment includes a separation cylinder 1 , a separation screen 2 , a valve 3 and a pipeline 4 . The separation screen 2 includes a first separation screen 21 , a second separation screen 22 and a third separation screen 23 which are arranged to sealably engage with the inner wall of the separation cylinder 1 from top to bottom. The first separation screen 21 is a nylon screen of 1200 μm, the second separation screen 22 is a nylon screen of 1000 μm, and the third separation screen 23 is a nylon screen of 500 μm. These three finenesses are obtained through repeated experiments. The best separation fineness can ensure the accuracy of separation. A water outlet is provided on the side wall of the separation cylinder 1 below the third separation screen 23 , and the valve 3 is arranged on the pipeline 4 communicating with the water outlet. The separation device of this em...

Embodiment 2

[0032] See figure 2 , the separation device of the large daphnia experiment for the female daphnia and young daphnia, including a separation cylinder 1, a separation screen 2 and a peristaltic pump 5. The separation screen 2 includes a first separation screen 21 and a second separation screen 22 that are sealed and engaged with the inner wall of the separation cylinder 1 from top to bottom, the first separation screen 21 is a nylon screen of 1200 μm, and the second The separation screen 22 is a 1000 μm nylon screen. The peristaltic pump 5 communicates with the mixed deluge liquid storage cylinder 7 and the separation cylinder 1 through the pipeline 4 . The 1L scale mark on the separation cylinder 1 is below the position of the second separation screen 22 . The separation principle of the separation device is the same as that of Embodiment 1. Because the peristaltic pump 5 is added, the mixed deluge fluid can be automatically drawn in volume, which improves the work efficien...

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Abstract

The invention discloses a device for separating mother daphnia magna and young daphnia magna for a daphnia magna experiment and a method of separating the mother daphnia magna and the young daphnia magna for the daphnia magna experiment. The device comprises a separating tank and separating screens, wherein the separating screens comprise a first separating screen and a second screen which are respectively stuck in the inner wall of the separating tank in a sealing way from top to bottom; the first separating screen is a nylon screen with the pore diameter of 1200 microns; and the second separating screen is a nylon screen with the pore diameter of 1000 microns. According to the device for separating the mother daphnia magna and the young daphnia magna for the daphnia magna experiment, the separating screens which are stuck in the separating tank in a sealing way are adopted, and optimal fineness is selected, so that the mother daphnia magna and the young daphnia magna for the daphnia magna experiment can be separated rapidly, conveniently and cleanly, and the mother daphnia magna and the young daphnia magna can be directly used in a laboratory or on the spot.

Description

technical field [0001] The invention relates to a separation device for experimental organisms, in particular to a separation device and a separation method for daphnia magna and juvenile daphnia used in experiments. Background technique [0002] Daphnia magna (Daphnia magna) is a creature living in natural waters, belonging to plankton, zooplankton, arthropods, crustaceans, subclass Branchopods, bicaria, Daphnia superfamily, Daphnia genus . Because of its short life cycle, rapid reproduction, economical, convenient and easy to obtain, easy to cultivate in the laboratory, etc., and its important position in the aquatic ecosystem, it has been valued by toxicologists in many countries. Daphnia magna is a highly sensitive and very representative zooplankton. It has become a standard test organism and is widely used in aquatic toxicology research. At present, ISO standards, OECD guidelines, US EPA standards and my country's national standards all have standards related to grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 薛银刚曹志俊蔡焕兴徐东炯王延军陈桥
Owner CHANGZHOU ENVIRONMENTAL MONITORING CENT
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