Device for separating mother daphnia magna and young daphnia magna for daphnia magna experiment and method of separating mother daphnia magna and young daphnia magna for daphnia magna experiment

Abstract

The invention discloses a device for separating mother daphnia magna and young daphnia magna for a daphnia magna experiment and a method of separating the mother daphnia magna and the young daphnia magna for the daphnia magna experiment. The device comprises a separating tank and separating screens, wherein the separating screens comprise a first separating screen and a second screen which are respectively stuck in the inner wall of the separating tank in a sealing way from top to bottom; the first separating screen is a nylon screen with the pore diameter of 1200 microns; and the second separating screen is a nylon screen with the pore diameter of 1000 microns. According to the device for separating the mother daphnia magna and the young daphnia magna for the daphnia magna experiment, the separating screens which are stuck in the separating tank in a sealing way are adopted, and optimal fineness is selected, so that the mother daphnia magna and the young daphnia magna for the daphnia magna experiment can be separated rapidly, conveniently and cleanly, and the mother daphnia magna and the young daphnia magna can be directly used in a laboratory or on the spot.

Description

technical field [0001] The invention relates to a separation device for experimental organisms, in particular to a separation device and a separation method for daphnia magna and juvenile daphnia used in experiments. Background technique [0002] Daphnia magna (Daphnia magna) is a creature living in natural waters, belonging to plankton, zooplankton, arthropods, crustaceans, subclass Branchopods, bicaria, Daphnia superfamily, Daphnia genus . Because of its short life cycle, rapid reproduction, economical, convenient and easy to obtain, easy to cultivate in the laboratory, etc., and its important position in the aquatic ecosystem, it has been valued by toxicologists in many countries. Daphnia magna is a highly sensitive and very representative zooplankton. It has become a standard test organism and is widely used in aquatic toxicology research. At present, ISO standards, OECD guidelines, US EPA standards and my country's national standards all have standards related to grow...

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Problems solved by technology

[0004] The deficiencies of the separation method of the above-mentioned national standard are: 1) in the standard method, the shelling produced by the reproduction of Daphnia magna and the algae pellets fed are not removed, and these substances will adhere to the body of Daphnia magna, causing the Movement is affected, which affects the normal growth and reproduction of Daphnia magna

2) The standard method adopts the nesting method to carry out the actual operation process, which is more cumbersome, and the screens of different sizes must be backwashed every time, and the separation speed is relatively slow

3) The automation degree of the standard method is not high enough to meet the large-scale and high-throughput operations in the future

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