High-density fermentation culture medium for haemophilus parasuis and preparation method for same

A high-density fermentation technology of Haemophilus suis, which is applied in the high-density fermentation medium of Haemophilus parasuis and its preparation field, can solve the problems of high medium cost, shortened culture time, and poor effect, and achieve cost reduction, low cost effect

Inactive Publication Date: 2013-08-07
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to screen out a high-performance fermentation medium for the defects of high cost and poor effect of the existing culture medium, which can not only ensure the vigorous growth of Haemophilus parasuis, but also increase the growth rate , shorten the culture time, and can be used for large-scale production of Haemophilus parasuis vaccine

Method used

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  • High-density fermentation culture medium for haemophilus parasuis and preparation method for same
  • High-density fermentation culture medium for haemophilus parasuis and preparation method for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of medium:

[0022] (1) Take yeast powder 10g / L, soybean peptone 1g / L, NaCl 2g / L, K 2 HPO 4 1g / L, dissolved in distilled water and adjusted to 1000ml, adjusted the pH of the medium to 7.0~7.4, and sterilized under high-pressure steam at 115~121°C for 20~30min;

[0023] (2) Take 1~10g of maltose, add distilled water to make up to 1000ml, make maltose solution, and sterilize under high-pressure steam at 115~121℃ for 20~30min;

[0024] (3) According to the volume ratio, take 1% of the maltose solution in step B, filter sterilized bovine serum 2%, NAD 0.001%, and the balance is the sterilized medium of step A to obtain Haemophilus parasuis High-density fermentation medium.

Embodiment 2

[0025] Embodiment 2: the preparation of optimization medium

[0026] (1) Take yeast powder 16g / L, soybean peptone 5g / L, NaCl 5g / L, K 2 HPO 4 5g / L, dissolved in distilled water and adjusted to 1000ml, adjusted the pH of the culture medium to 7.0~7.4, and sterilized under high-pressure steam at 115~121°C for 20~30min;

[0027] (2) Take 1~10g maltose, add distilled water to make up to 1000ml, and make maltose

[0028] Solution, sterilized under high pressure steam at 115~121℃ for 20~30min;

[0029] (3) According to the volume ratio, take 5% of the maltose solution in step B, 5% of filter-sterilized bovine serum, and 0.005% of NAD, and the balance is the sterilized medium of step A to obtain Haemophilus parasuis High-density fermentation medium.

Embodiment 3

[0030] Embodiment 3: the preparation of optimization medium

[0031] (1) Take yeast powder 30g / L, soybean peptone 10g / L, NaCl 10g / L, K 2 HPO 4 10g / L, dissolved in distilled water and adjusted to 1000ml, adjusted the pH of the medium to 7.0~7.4, and sterilized under high-pressure steam at 115~121°C for 20~30min;

[0032] (2) Take 1~10g of maltose, add distilled water to make up to 1000ml, make maltose solution, and sterilize under high-pressure steam at 115~121℃ for 20~30min;

[0033] (3) According to the volume ratio, take 10% of the maltose solution in step B, 10% of filter-sterilized bovine serum, and 0.01% of NAD, and the balance is the sterilized medium of step A to obtain Haemophilus parasuis High-density fermentation medium.

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Abstract

The invention belongs to the technical field of agriculture microbes, and provides a high-density fermentation culture medium for haemophilus parasuis and a preparation method for the same. The culture medium comprises the following components in weight / volume: 10-30 g / L of yeast powder, 1-10 g / L of soybean peptone, 1-10 g / L of maltose, 2-10 g / L of NaCl and 1-10 g / L of K2HPO4, the following components in volume ratio: 0.001-0.010% of NAD (nicotinamide adenine dinucleotide) and 2-10% of bovine serum, and the balance of water, wherein the pH is 7.0-7.4. The culture medium disclosed by the invention is beneficial to high-density fermentation for haemophilus parasuis, capable of obtaining a high bacterial quantity in a short time, low in cost, and capable of being used for large-scale production for vaccines.

Description

technical field [0001] The invention relates to a high-density fermentation medium of Haemophilus parasuis and its preparation, belonging to the technical field of agricultural microorganisms. Background technique [0002] Haemophilus parasuis disease, also known as porcine Glasser's disease, fibrinous serositis and arthritis, is a Gram-negative small bacillus, showing sporadic polyserositis and arthritis. With the development of the pig industry, the application of large-scale feeding technology and high-intensity feeding, and the existence of factors such as sudden new respiratory syndromes, the disease is becoming more and more popular and the harm is becoming more and more serious. In the past two years, there have been frequent reports of porcine polyserositis and arthritis caused by Haemophilus parabacterium in pig farms in my country, especially when the immune function of large-scale pig farms is reduced after being infected by blue ear disease and circus disease. Th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/21
Inventor 单虎段笑笑韩先杰张传美秦志华蔡秀磊
Owner QINGDAO AGRI UNIV
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