Supercharge Your Innovation With Domain-Expert AI Agents!

Magnetic bead method for quickly purifying antibody

A magnetic bead method, magnetic bead technology, applied in organic chemistry, peptide preparation methods, chemical instruments and methods, etc.

Inactive Publication Date: 2013-09-04
HENAN UNIVERSITY OF TECHNOLOGY
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Commonly used methods for antibody purification include caprylic ammonium sulfate precipitation, ion exchange chromatography and SPA affinity chromatography. A large number of chemical reagents and experimental instruments (such as centrifuges, purification chromatography workstations, etc.), preparation of various buffer solutions, and multi-step operations (such as centrifugation, precipitation, chromatography purification, etc.) are required. The whole process takes a long time and is effective. During working hours, the method of human operation or machine purification, the number of purified samples is limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Magnetic bead method for quickly purifying antibody
  • Magnetic bead method for quickly purifying antibody
  • Magnetic bead method for quickly purifying antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Purification of Antibodies from Human Whole Blood and Pig Serum by Protein A Magnetic Beads

[0037] 1. Antibody purification

[0038] (1) Take 0.5mL of human whole blood (or 0.1mL of pig serum) into a 2mL EP tube;

[0039] (2) Dilute human whole blood 1-3 times with pH 7.4 PBS buffer;

[0040] (3) Add 50uL Protein A magnetic beads (vortex or pipette to mix the magnetic beads before use);

[0041] (4) Vortex for 10s, incubate at room temperature for 10min (during this period, vortex twice);

[0042] (5) Place the centrifuge tube on the magnetic stand, suck off the supernatant after 1 min (the centrifuge tube is kept on the magnetic stand), and be careful not to attract the magnetic beads;

[0043] (6) Add 500uL pH 7.4 PBS buffer, vortex for 5s, and wash the ProteinA magnetic beads;

[0044] (7) Place the centrifuge tube on the magnetic stand, suck off the supernatant after 1 min (the centrifuge tube is kept on the magnetic stand), and be careful not to ...

Embodiment 2

[0065] Example 2: Purification of antibodies from pig serum (1 mL) by Protein A magnetic beads—consecutive use for multiple times

[0066] 1. Antibody purification

[0067] (1) Take 0.2mL of pig serum into a 2mL EP tube;

[0068] (2) Dilute 1-3 times with pH 7.4 PBS buffer;

[0069] (3) Add 50uL ProteinA magnetic beads (vortex or use a pipette to mix the magnetic beads before use);

[0070] (4) Vortex for 10s, incubate at room temperature for 10min (during this period, vortex twice);

[0071] (5) Place the centrifuge tube on the magnetic stand, suck off the supernatant after 1 min (the centrifuge tube is kept on the magnetic stand), and be careful not to attract the magnetic beads;

[0072] (6) Add 500uL pH 7.4 PBS buffer, vortex for 5s, and wash the ProteinA magnetic beads;

[0073] (7) Place the centrifuge tube on the magnetic stand, suck off the supernatant after 1 min (the centrifuge tube is kept on the magnetic stand), and be careful not to attract the magnetic bead...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the biological and pharmaceutical industry, and discloses a magnetic bead method for quickly purifying an antibody. The method is characterized in that by using an affinity chromatography principle, carboxyl magnetic beads are activated, and then connected with stphylococcl protein A (SPA) capable of being combined with Fc (fragment crystalline) of IgG (intravenous gamma globulin) molecules of human and various mammals; and obtained Protein A magnetic beads can be used for purifying the antibody in serum, ascites or a cell culture fluid. The Protein A magnetic beads are high in specificity, quick and convenient in the aspect of antibody purification, and can be widely applied to the biological and pharmaceutical industry.

Description

technical field [0001] The invention relates to biological and pharmaceutical industries, and can more conveniently and quickly purify IgG class antibodies from animal ascites, serum or cell culture. Background technique [0002] Commonly used methods for antibody purification include caprylic ammonium sulfate precipitation, ion exchange chromatography, and SPA affinity chromatography. These methods require a large number of chemical reagents and experimental instruments (such as centrifuges, purification chromatography, etc.) in the process of antibody purification. Workstation, etc.), prepare a variety of buffers, and perform multi-step operations (such as centrifugation, precipitation, chromatographic purification, etc.), the whole process takes a long time, and within the effective working time, the method of human operation or machine purification, its purification Sample size is limited. Compared with the traditional purification method, the magnetic bead method has o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/00C07K1/14
Inventor 李华梁永波陆启玉
Owner HENAN UNIVERSITY OF TECHNOLOGY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com