Drum type biochip spotting device and method thereof

Abstract

The invention relates to a drum type biochip spotting device and a method thereof. The device comprises: a drum having an outer circumference surface, wherein a biochip matrix can be coated on the outer circumference surface of the drum; a drum driving mechanism used for rotating the drum; a spotting needle rack arranged over the drum and provided with a spotting needle array; and a spotting needle rack driving mechanism used for making the spotting needle rack move relative to the drum. The device uses the surface of the drum as the bearing surfaces of biochips in the invention, so the space utilization rate is substantially increased, a spotting pipeline is shortened, and the bubble generation possibility is reduced. A slide block provided with a spotting needle does not move in the vertical spotting process, so a pipeline system is always in a stationary state, and the pressure change influencing the spotting precision is very small, thereby the spotting precision and the spotting efficiency are greatly increased.

Description

Technical field [0001] The invention relates to a biochip spotting device and method. Background technique [0002] In many aspects, such as deciphering the mystery of human genetics and drug screening, the biochip spotting instrument (or “spotting device”) has become an indispensable basic equipment. Therefore, the technical level of the biochip spotter and the performance indicators also affect the process of life science research to some extent. In terms of function only, no matter what kind of biochip spotting instrument, it is to be able to study or test DNA, RNA, polypeptide, protein and other biological components according to the desired matrix point with high density and high precision It is fixed on a solid-phase dense carrier such as a silicon wafer, a glass wafer or a ceramic wafer to form a biomolecular lattice. [0003] As the backing and executor of precision, speed, function, etc., mechanical structure plays a pivotal role in the biochip spotter. A reasonable mec...

AI Technical Summary

Problems solved by technology

Such a mechanical structure layout is relatively complicated and loose, and the movement range of the slide table carrying the biochip (substrate) is large, and the space utilization rate is not high.

The spotting instrument of this structure occupies a large area in use, and because the spotting pipeline of the spotting needle (in the existing spotting instrument, the lengthy length of bending is usually used between the spotting needle and the sample container). The pipeline is connected, which can be referred to as "sampling pipeline" for short) is very long, and the possibility of air bubbles is relatively high during sample application

In addition, during the sampling process, the sampling needle needs to move continuously, the pipeline system is also unstable, and the pressure change that affects the sampling accuracy is relatively large; this in turn leads to low sampling efficiency, low sampling accuracy, and biochip processing The time is relatively long, which makes it difficult for most scientific research institutions to accept

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