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Production method of fritillaria hupehensis seed ball

A production method and technology of Fritillaria, applied in the production field of Hubei Fritillary bulbs, can solve the problems of high cost, low output, slow output, etc., to improve yield and quality, high transplant survival rate, and improve economic benefits Effect

Active Publication Date: 2013-09-11
RES INST OF TRADITIONAL CHINESE MEDICINE PLANTS HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the defects of high cost, slow output, and low output in artificial planting, and provide an artificial planting technology that can not only improve economic benefits per unit area, stabilize production, but also improve output and quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for inducing test-tube bulbs of Fritillaria, comprising the following steps:

[0026] a. Preparation of culture medium

[0027] Callus induction medium: MS+ 6-BA 3mg / L+NAA 0.2mg / L+sucrose 30g / L+agar 6-6.5g / L, pH 5.9-6.0;

[0028] Callus proliferation medium: MS+ 6-BA 2mg / L+NAA 0.2mg / L+sucrose 30g / L+agar 6-6.5g / L, pH value 5.9-6.0;

[0029] Callus differentiation medium: MS+ 6-BA 2mg / L+NAA 0.2mg / L+sucrose 30g / L+agar 6-6.5g / L, pH value 5.9-6.0;

[0030] Tube bulb induction medium: 1 / 2MS+NAA 1.0mg / L+sucrose 30g / L+agar 6-6.5g / L, pH 5.9-6.0;

[0031] b. Induction of Hubei Fritillaria in vitro bulbs

[0032] Selection of explants and surface disinfection: In spring, at the peak of the emergence of Fritillaria, select 4 full bulbs without pests and diseases, store them at 4°C for about a week, take out and wash the surface soil, remove the old bulbs on the outer layer, and remove the old bulbs from the outer layer. The tender bulbs were used as explants, soaked in...

Embodiment 2

[0040] A method for inducing test-tube bulbs of Fritillaria, comprising the following steps:

[0041] a. Preparation of culture medium

[0042] Callus induction medium: MS+ 6-BA 2mg / L+ NAA 0.2mg / L+ sucrose 20g / L+ agar 6-6.5g / L, pH value 5.9-6.0;

[0043] Callus proliferation medium: MS+ 6-BA 2mg / L+ NAA 0.5mg / L+ sucrose 30g / L+ agar 6-6.5g / L, pH value 5.9-6.0;

[0044] Callus differentiation medium: MS+ 6-BA 3mg / L+ NAA 0.2mg / L+ sucrose 30g / L+ agar 6-6.5g / L, pH value 5.9-6.0;

[0045] Tube bulb induction medium: 1 / 2MS+NAA 0.5mg / L+sucrose 30g / L+agar 6-6.5g / L, pH 5.9-6.0;

[0046] b. Induction of Hubei Fritillaria in vitro bulbs

[0047] Selection of explants and surface disinfection: In spring, at the peak of the emergence of Fritillaria, select 4 full bulbs without pests and diseases, store them at 4°C for about a week, take out and wash the surface soil, remove the old bulbs on the outer layer, and remove the old bulbs from the outer layer. The tender bulbs were used as expl...

Embodiment 3

[0055] a. Preparation of culture medium

[0056] Callus induction medium: MS+ 6-BA 3mg / L+ NAA 1mg / L+ sucrose 20g / L agar 6-6.5g / L, pH 5.9-6.0;

[0057] Callus proliferation medium: MS+ 6-BA 3mg / L+ NAA 1mg / L+ sucrose 20g / L+ agar 6-6.5g / L, pH 5.9-6.0;

[0058] Callus differentiation medium: MS+ 6-BA 2mg / L+NAA 0.2mg / L+sucrose 30g / L+agar 6-6.5g / L, pH value 5.9-6.0;

[0059] Tube bulb induction medium: 1 / 2MS+NAA 1.0mg / L+sucrose 30g / L+agar 6-6.5g / L, pH 5.9-6.0;

[0060] b. Induction of Hubei Fritillaria in vitro bulbs

[0061] Selection of explants and surface disinfection: In spring, at the peak of the emergence of Fritillaria, select 4 full bulbs without pests and diseases, store them at 4°C for about a week, take out and wash the surface soil, remove the old bulbs on the outer layer, and remove the old bulbs from the outer layer. The tender bulbs were used as explants, soaked in 75% alcohol for 30-60s, soaked in 0.1% mercury chloride aqueous solution for 8min, and then rinsed w...

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Abstract

The invention relates to a production method of a fritillaria hupehensis seed ball. The production method is characterized by comprising the following operation steps: a. preparing a culture medium; b. inducing a fritillaria hupehensis test tube bulb: selecting an explant and disinfecting a surface; inducing a callus; proliferating the callus; differentiating the callus; inducing the test tube bulb; c. cultivating the fritillaria hupehensis seed ball. Compared with the prior art, the production method has the advantages that the period of producing the fritillaria hupehensis seed ball is 6-9 months so that the growth period of fritillaria is shortened to 1 year from 2-3 years; the propagation coefficient can be up to be more than 1000 times; the fritillaria hupehensis seed ball produced by the method disclosed by the invention is high in survival rate, and the non-toxic high-quality seed ball can be provided for production.

Description

technical field [0001] The invention relates to a method for producing Hubei Fritillaria seed bulbs. Background technique [0002] Hubei Fritillaria (Fritillaria hupehensis Hsiao et K.C.Hsia), also known as Ebe, Banbei, Yaobei, Fengbei, is mainly distributed in western and southwestern Hubei, and also has a small distribution in eastern Sichuan, northwestern Hunan, Anhui, and Henan. Folk study Chuanbei medicinal. Hubei fritillary mainly contains steroidal alkaloids, and the total alkaloid content is higher than that of Sichuan fritillary and Zhejiang fritillary, and fritillary A can be regarded as a characteristic component of fritillary Hubei, and its content is determined at 0.04330%-0.09016%. In the 2000 and 2005 editions of "Chinese Pharmacopoeia", it has become the second largest mainstream Fritillaria product in the Fritillaria market. By the end of 2008, the preserved area of ​​Fritillaria in Hubei Province, Enshi Prefecture was 9468.7 mu. As one of the key varietie...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王华廖朝林何美军穆森张宇张美德廖璐婧
Owner RES INST OF TRADITIONAL CHINESE MEDICINE PLANTS HUBEI ACAD OF AGRI SCI
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