Unlock instant, AI-driven research and patent intelligence for your innovation.

Plant minichromosome and its preparation method and use

A minichromosome and plant technology, applied in the fields of molecular biology and genetic engineering, can solve problems such as no in vivo verification

Active Publication Date: 2016-01-06
THE CHINESE UNIVERSITY OF HONG KONG +1
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lin et al. have constructed vectors for the de novo construction of rice minichromosomes (Lin et al., 2011), but have not yet performed in vivo verification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant minichromosome and its preparation method and use
  • Plant minichromosome and its preparation method and use
  • Plant minichromosome and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 produces the plasmid construction of minichromosome

[0071] figure 1 Maps of the three plasmids used to make plant minichromosomes are shown. 4T3X contains Arabidopsis thaliana telomeres (about 1.3kb) cloned with three direct repeats, which can induce the production of new telomeres after the plasmid integrates into the chromosome; pED97 contains the Cre-lox recombination system, which can endow minichromosomes with new genetic capacity. pCAMBIA1301 contains the HptII gene, which can make the transformed rice cells resistant to hygromycin, and it also contains the GUS gene, and the transformed cells can turn blue after histochemical staining. figure 1 Among them, P35S refers to CaMV35S promoter; HptII refers to hygromycin phosphotransferase II gene; T35S refers to CaMV35S terminator; GUS refers to β-glucuronidase gene; Tnos refers to nopaline synthase gene terminator; Telo refers to Arabidopsis Telomere repeat; lox66 refers to lox66 recombination site; ...

Embodiment 2

[0072] Example 2 Utilizes plasmid bombardment of rice callus

[0073]We mixed three plasmids and used them for bombardment of rice calli: 4T3X plasmid contains about 1.3kb Arabidopsis telomere sequence; pCAMBIA1301 contains hygromycin resistance gene and GUS reporter gene used as selectable marker; pED97 contains Cre / lox site-specific recombination system, through which new genes can be added to the minichromosome. Each part of 2 micrograms of the above-mentioned purified plasmid DNA was mixed 5:1:1 and adhered to 1.5 mg of 0.6 micron gold powder particles. The rice callus was bombarded with a PDS1000 / He type gene gun of BIO-RAD Company with a pressure of 650PSI. After transformation, calli were cultured on medium containing 25 mg / L hygromycin for two weeks and then transferred to selection medium containing 50 mg / L hygromycin for selection to allow growth of resistant calli . Hygromycin-resistant clones were detected by FISH using the transgene as a probe to detect chromos...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for producing a minute chromosome in a plant cell, which comprises a step of converting a plant cell by use of a first carrier containing telomeric repeats and a second carrier containing recombination sites. The invention also provides a method for preparing separated minute chromosomes and a method for preparing transgenic plants.

Description

field of invention [0001] This application relates generally to the fields of molecular biology and genetic engineering. More specifically, the present application relates to plant minichromosomes and their preparation methods and uses. Background of the invention [0002] When transforming plants with traditional Agrobacterium or gene gun methods, the number of genes that can be transferred at the same time is very limited, only one to several. Using improved methods such as BIBAC (Hamiltone et al., 1996; Vega et al., 2008) or TAC (Liu et al., 1999), fragments as long as 80 to 150 kb can be transformed into plants, but multiple genes are constructed on such a large vector There are still many difficulties. In order to integrate multiple genes together, the usual strategy is to transform each gene separately and then integrate them in later breeding. Therefore, it takes many years to complete the integration of multiple genes in one germplasm using traditional genetic bre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/10C12N5/04C12N15/05A01H5/00
Inventor 徐春晖于为常
Owner THE CHINESE UNIVERSITY OF HONG KONG
PatSnap Eureka logo

PatSnap Eureka turns technology decisions into work you can execute. Powered by our Innovation Knowledge Graph, it runs expert workflows across engineering, life sciences, materials and intellectual property. Get your review-ready output in minutes.

X (Twitter)LinkedInYouTubeSubstack

© 2026 PatSnap. All rights reserved. 

Terms of Service

 | 

Privacy policy

 | 

Modern Slavery Act Transparency Statement