A Synthetic Aperture Microscopy Device Based on Light Field Selection

Abstract

The invention discloses a synthetic aperture microscopy method on the basis of light field selection. The synthetic aperture microscopy method includes steps of (1) after processing probe light by means of expanding and deflecting, irradiating the probe light to a sample and collecting transmission light penetrates the sample by the aid of a microscopy imaging system, (2) combining and polarizing uniformly reference light with the transmission light collected by the microscopy imaging system after processing the reference light by means of expanding and deflecting, and recording an interference pattern of the two light beams through an image sensor, (3) changing irradiating angle of the probe light to the sample, repeating the steps (1) and (2) to acquire multiple interference patterns corresponding to multiple angles, and (4) obtaining phase distribution of each interference pattern by means of Fourier transform, and finally reconstructing to obtain microscopy images by a randon algorithm. The invention further discloses a synthetic aperture microscopy device on the basis of light field selection. By the synthetic aperture microscopy method with the light field selection, synthetic aperture of a microscope is increased effectively, lateral resolution and longitudinal resolution are improved, and super resolution effect is achieved.

Description

technical field [0001] The invention belongs to the field of optical super-resolution microscopy, in particular to a synthetic aperture microscopy method and device based on light field selection. Background technique [0002] The 21st century is a period of rapid development of biological information technology. In this process of continuous development, the demand for the observation of the subcellular structure of living cells is becoming more and more urgent. First of all, in order to realize the observation of the original living state of living cells, the use of dyes should be avoided as much as possible. At the same time, since most biological samples are colorless and transparent, phase measurement is a commonly used method at present. Since the microscopic objective lens itself is limited to only receive low-frequency information of the sample light, how to expand the numerical aperture of the microscopic objective lens is a problem. An important way to improve res...

AI Technical Summary

Problems solved by technology

But the problem is that the two-dimensional scanning galvanometer has no stable fixed point during the scanning process, so a certain systematic error will inevitably be introduced in the process of synthesizing the aperture.

Their scheme introduces a certain tilt angle in principle when the wedge prism rotates, and because the wedge angle of the wedge prism is fixed, it lacks adjustability in the process of synthesizing the aperture

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