Detection kit for myeloperoxidase content and preparation method thereof

Abstract

The invention discloses a detection kit for myeloperoxidase content and a preparation method thereof. The detection kit comprises reagent I and reagent II which are independent of each other, wherein the reagent I comprises a biological buffer, a surfactant, a coagulation accelerator, an antiseptic, a stabilizer, a blocker, a chelating agent and water; and the reagent II comprises latex particles which encapsulate antibodies of the myeloperoxidase, a biological buffer, a chelating agent, a surfactant, an antiseptic, a suspending agent, a blocking agent, a stabilizer and water. The detection kit for myeloperoxidase content has advantages that: stability, sensitivity and specificity are high; detecting operation is easy; and the kit is suitable for large-scale popularization and application.

Description

technical field [0001] The present invention relates to a detection kit for the content of myeloperoxidase in the human body and a preparation method thereof. The present invention also relates to a method for using the myeloperoxidase determination kit in detecting the content of myeloperoxidase in the human body. The field of detection of myeloperoxidase content in human body. Background technique [0002] Myeloperoxidase (MPO), also known as peroxidase, is an important iron-containing lysosome that exists in the azurophilic granules of myeloid cells (mainly neutrophils and monocytes) , is a specific marker of myeloid cells. Myeloperoxidase (MPO) is a heme protease containing heme prosthetic group secreted by neutrophils, monocytes and macrophages of certain tissues, and is a member of the heme peroxidase superfamily . MPO is a phase I metabolic enzyme. There are two ferrite groups per enzyme molecule, and paramagnetic resonance spectroscopy shows that the iron in heme...

AI Technical Summary

Problems solved by technology

Enzyme-linked immunoassay is not highly automated, and the detection operation is cumbersome, time-consuming, and greatly affected by human factors; double-antibody sandwich photochemiluminescence immunoassay requires special instruments, and the detection cost is high; The specificity is poor, these factors limit the large-scale application of these detection methods

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