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Compound fermentation formula and technology of compound amino acid purine mother liquor Niannianle

An amino acid purine and compound fermentation technology, which is applied in compound amino acid purine mother liquor-Niannianle compound fermentation formula and process field, can solve the problems of backward fermentation production process, single fermentation strain and low yield, etc.

Inactive Publication Date: 2013-10-02
唐睿
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention solves the problems that the fermentation production process is relatively backward, the fermentation strain is relatively single, the fermentation substrate cannot be well utilized, and the yield is low in the current microbial fermentation production process of cytokinin.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0010] Example 1: Preparation of 5% compound amino acid purine mother solution: the formula of compound medium is KNO 3 -1g, K 2 HPO 4 -0.5g, NaCl -0.5g, FeSO 4 ·7H 2 O-0.01g, soluble starch-20g, MgSO 4 ·7H 2 O-0.5g, MnSO 4 ·H 2 O-0.05g, glycerol-3g, 0.2% polyether modified silicone oil as defoaming agent, pH value adjusted to 6.5-7.2, after the compound medium was prepared according to the above formula, pour it into the conical shaker flask, after 121 ℃ autoclave for 30 minutes, after cooling, put three strains of ACCC40027 Streptomyces luteus, ACCC40822 Streptomyces light flavinus and ACCC40024 Streptomyces lavender gray on the ultra-clean workbench under the condition of aseptic operation Each of them was made into a bacterial solution, which was inoculated into an Erlenmeyer shaker flask respectively, and the inoculation ratio of each bottle was 3%-8%. Mix and shake at a ratio of 1:1:1, and then inoculate into a fermenter at a ratio of 3%-8%.

[0011] Fermentatio...

example 2

[0013] Example 2: Preparation of 8% compound amino acid purine mother liquor

[0014] According to the method of example 1, the fermenter nutrient solution formula is changed into 12% corn flour, 12% soybean flour, 6% anhydrous glucose, 6% maltose, 0.08% MnSO 4 ·H 2 O, 0.3% glycerin, add 0.35% polyether modified silicone oil as defoamer, and ferment to obtain compound amino acid content greater than or equal to 8%, hydroxyene adenine content greater than or equal to 8mg / g, ene adenine content Greater than or equal to 0.8mg / g compound preparation of the present invention, dilute 1500 times, use clear water as contrast, every kind of medicament design 4 repeats, before 10 o'clock in the morning or after 4 o'clock in the afternoon, spray to the seedling stage, Spraying is carried out at the early flowering stage, the late flowering stage and the fruit swelling stage, and tests show that the preparation can increase the grape yield by more than 28%.

example 3

[0015] Example 3: Preparation of 13% compound amino acid purine mother liquor

[0016] According to the method of example 1, the fermenter nutrient solution formula is changed into 15% corn flour, 15% bean flour, 8% anhydrous glucose, 8% maltose, 0.1% MnSO 4 ·H 2 O, 0.3% glycerin, add 0.5% polyether modified silicone oil as defoamer, and ferment to obtain compound amino acid content greater than or equal to 13%, hydroxyene adenine content greater than or equal to 13mg / g, ene adenine content Greater than or equal to 1.3mg / g compound preparation of the present invention, after diluting 2000 times, use clear water as contrast, every kind of medicament design 4 repeats, before 10 o'clock in the morning or after 4 o'clock in the afternoon, use sprayer to the various period of the cucumber of morbidity. Spraying is carried out, and the test shows that the increase of cucumber yield by the preparation reaches more than 33%.

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PUM

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Abstract

The invention relates to a compound fermentation technology and a formula of a compound amino acid purine mother liquor. Fermentation microbes belong to the group of Actinomycetes, and are Streptomyces microflavus, Streptomyces flaveolus and Streptomyces lavendulae. The mother liquor comprises, by weight: 0.1 to 30% of compound amino acid, 0.1 to 30mg / g of oxyenadenine, and 0.1 to 30mg / g of enadenine.

Description

technical field [0001] The invention relates to a compound fermentation production (by weight percentage) of 0.1%-30% compound amino acid and hydroxyene by using three strains of actinomycetes Streptomyces microflavus, Streptomyces flaveolus and Streptomyces lavendulae Process and formulation of adenine 0.1mg / g-30mg / g and ene adenine mother liquor. Background technique [0002] Hydroxyene adenine and ene adenine are cytokinins, one of the five endogenous plant hormones, which can promote cell division and growth of crops, improve crop yield and quality, and have been used more and more in agriculture in recent years. However, the content of hydroxyene adenine and ene adenine in plants is very low, and they are generally extracted from seaweed by chemical synthesis, but the process is complicated, the yield is low, the production cost is large, and the production scale cannot satisfy people in agriculture. Production and living needs. [0003] The present invention uses thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P21/00C12P17/18C12P13/00C12R1/465C12R1/56
Inventor 任承才唐睿韩铁冰吴景霄杨照东莫秋菊
Owner 唐睿
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