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Culture medium and culture method for producing sanglifehrin through fermentation of streptomyces flaveolus

A light yellow Streptomyces sp. and fermentation medium technology, applied in the field of fermentation, can solve the problems of autolysis of bacteria, lower fermentation titer, easy discoloration of fermentation broth, etc., and achieve the effect of increasing fermentation units

Inactive Publication Date: 2015-08-19
NINGXIA TAIYICIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]3 Adsorption resin was added to the fermentation medium, which increased the cost of fermentation and extraction
[0007]4 The procurement cost of raw materials required for sanglifehrin fermentation production is relatively high, especially the market price of organic nitrogen source yeast extract commonly used in sanglifehrin medium Higher, thereby increasing the fermentation cost and reducing market competitiveness
[0008]5 In the late stage of sanglifehrin fermentation and cultivation, bacterial autolysis is easy to occur, which reduces the fermentation titer
[0009]6 Amino acids are added to the fermentation medium, and the color of the fermentation broth is easy to change, which increases the difficulty of extraction and purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Seed culture: seed medium volume 10m 3 .

[0077] Seed medium: 10L peanut oil, 50kg maltose, 10g maltase, 110kg earthworm powder, 100L corn steep liquor, 50kg biological nitrogen, 5kg ammonium nitrate, 10kg light calcium carbonate; 1kg potassium dihydrogen phosphate.

[0078] Firstly, the seed medium is sterilized and cooled to 25-30°C, and kept under pressure with sterile air. After testing, the amino nitrogen is 31mg / 100ml, the dissolved phosphorus is 32ug / ml, the total sugar is 11mg / 100ml, and the pH is 6.2. Under flame protection, transfer the fermented liquid from the mother bottle of Streptomyces flavus into the seed medium, and the amount of inoculation is 1 L. Seed culture conditions are tank pressure 0.03-0.05MPa; tank temperature 24-26°C; air flow: 0-10h, using air stirring instead of mechanical stirring; 11h-transplanting: 20m 3 / h; stirring speed 60r / min. After the seed cultivation is completed, the cell concentration is 20%, the pH value is 6.9, and the...

Embodiment 2

[0082] Seed culture: seed medium volume 10m 3 .

[0083] Peanut oil 20L, maltose 60kg, maltase 20g, earthworm powder 120kg, corn steep liquor 110L, biological nitrogen 60kg, ammonium nitrate 6kg, light calcium carbonate 20kg; potassium dihydrogen phosphate 2kg. Firstly, the seed medium is sterilized and cooled to 25-30°C, and kept under pressure with sterile air. After testing, the amino nitrogen is 35mg / 100ml, the dissolved phosphorus is 34ug / ml, the total sugar is 13mg / 100ml, and the pH is 6.3. Under flame protection, transfer the Streptomyces flavum mother bottle fermentation liquid into the seed medium, and the inoculation volume is 1.2 L. Seed culture conditions are tank pressure 0.03-0.05MPa; tank temperature 24-26°C; air flow: 0-10h, using air stirring instead of mechanical stirring; 11h-transplanting: 25m 3 / h; stirring speed 65r / min. After the seed cultivation is completed, the cell concentration is 23%, the pH value is 6.7, and the cultivation time is 45h.

[0...

Embodiment 3

[0087] Seed culture: seed medium volume 10m 3 .

[0088] Peanut oil 30L, maltose 70kg, maltase 30g, earthworm powder 130kg, corn steep liquor 120L, biological nitrogen 70kg, ammonium nitrate 7kg, light calcium carbonate 30kg; potassium dihydrogen phosphate 3kg. Firstly, the seed medium is sterilized and cooled to 25-30°C, and kept under pressure with sterile air. After testing, the amino nitrogen is 41mg / 100ml, the dissolved phosphorus is 39ug / ml, the total sugar is 15mg / 100ml, and the pH is 6.5. Under flame protection, transfer the fermented liquid of the mother bottle of Streptomyces flavus into the seed medium, and the amount of inoculation is 1.5 L. Seed culture conditions are tank pressure 0.03-0.05MPa; tank temperature 24-26°C; air flow: 0-10h, using air stirring instead of mechanical stirring; 11h-transplanting: 30m 3 / h; stirring speed 70r / min. After the seed cultivation is completed, the cell concentration is 25%, the pH value is 6.5, and the cultivation time is ...

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Abstract

The invention relates to a culture medium and a culture method for producing sanglifehrin through the fermentation of streptomyces flaveolus. By using a formula of the culture medium and a fermentation control process, provided by the invention, the fermentation unit of sanglifehrin can be improved and the fermentation cost can be reduced; and the influence of environment on sources of raw materials and auxiliary materials is reduced to the minimum, and the adequate supply of the raw materials and auxiliary materials is ensured, thereby realizing the stable and efficient production of sanglifehrin.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a novel culture medium and a culture method for fermenting and producing sangliferin by Streptomyces pale flavum. Background technique [0002] Sanglifellin is a new type of macrolide antibiotic produced by Streptomyces, and it is currently a commonly used immunosuppressant. Sanglifehrin is mainly composed of 5 components, which are A, B, C, D and E components, and sanglifehrin A is the key component among them, and its content is relatively high. At present, the marketed immunosuppressive drugs have certain side effects of nephrotoxicity and central toxicity. Therefore, as a new generation of highly effective and low-toxic immunosuppressants, sanglifehrin has high drug development value and market prospects. [0003] At present, the fermentation production of sangliferoin in China adopts the secondary or tertiary fermentation mode. The main problems of this me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/465
Inventor 任勇
Owner NINGXIA TAIYICIN BIOTECH CO LTD
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