Nucleic acid aptamer specifically binding to human epidermal growth factor acceptor III type mutant and applications thereof

A technology of epidermal growth factor and nucleic acid aptamer, which is applied in the field of biomedicine, can solve the problems of drug resistance and side effects, and achieve the effect of broad application prospects
CN103333895AActive Publication Date: 2013-10-02SOUTHERN MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SOUTHERN MEDICAL UNIVERSITY
Publication Date
2013-10-02

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Abstract

The invention discloses a nucleic acid aptamer specifically binding to human epidermal growth factor acceptor III type mutant and applications thereof. The nucleotide sequence of the nucleic acid aptamer is shown as any one of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5. The nucleic acid aptamer is bound with only a U87[delta] cell line, and not bound with a U87 cell line or a HEK293 cell line, and therefore the nucleic acid aptamer can be effectively applied for preparing sensors or kits that diagnose tumor cells expressing EGFRvIII. In addition, the nucleic acid aptamer can be used as a carrier carrying an anti-EGFRvIII-expressed-tumor-cell medicine or siRNA to enter into the tumor cells expressing the EGFRvIII or tumor tissues expressing the EGFRvIII, and therefore the nucleic acid aptamer has a targeting function and a wide application prospect.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to a nucleic acid aptamer specifically combined with a human epidermal growth factor receptor type III mutant and an application thereof. Background technique

[0002] In recent years, some biomarkers for cancer diagnosis and treatment have been studied. Of these, cancer cell-specific membrane proteins are considered the most appropriate biomarkers, as they are often shed in detectable amounts in body fluids, and the clinical presentation of body fluids is often superior to invasive methods of biopsy sampling. In addition to this, membrane proteins have attracted much attention due to their potential use in cancer tissue imaging and targeted therapeutic strategies.

[0003] Meanwhile, the field of biomarker identification mainly relies on two-dimensional electrophoresis (2D-GE) and mass spectrometry. However, there are considerable limitations to elucidating membrane protein...

Claims

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