Bacterium capable of degrading herbicide acetochlor and application thereof

A technology of acetochlor and bacterial agents, applied in the field of bacteria that degrades the herbicide acetochlor, can solve environmental pollution, farmland soil and groundwater pollution, serious problems, etc.

Active Publication Date: 2013-10-09
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extensive use of herbicides has greatly saved labor costs and improved agricultural production efficiency, but it has also brought some ecological problems such as agricultural production and environmental pollution.
Acetochlor has a short half-life and has always

Method used

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  • Bacterium capable of degrading herbicide acetochlor and application thereof
  • Bacterium capable of degrading herbicide acetochlor and application thereof
  • Bacterium capable of degrading herbicide acetochlor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1, Isolation and Identification of Mycobacterium sp. YCA11CGMCC No.7771

[0039] 1. Isolation of acetochlor-degrading bacteria YCA11

[0040] Add 5g of soil sample (collected from the farmland polluted by the herbicide acetochlor in Beijing, China) to 50mL of the enriched culture solution with acetochlor concentration of 300mg / L, culture with shaking at 28°C and 200r / min for 7 days, and absorb 5mL of the culture solution Transfer to the enrichment medium with acetochlor concentration of 500mg / L, and continue to culture for 7 days, such continuous enrichment and culture for 5 times, the concentration of acetochlor is 300, 500, 700, 900 and 1100mg / L. After the enrichment is completed, take the enrichment solution with acetochlor concentration of 1100mg / L for serial dilution, spread evenly on the inorganic salt solid medium with the same concentration of acetochlor, and culture at 28°C. After a single colony trail appears on the plate, Pick a larger single colony ...

Embodiment 2

[0063] Example 2, Quantitative Determination of Acetochlor Degradation Ability of Mycobacterium sp. YCA11CGMCC No.7771

[0064] 1. Preparation of standard curve for acetochlor determination

[0065] The acetochlor standard (purchased from Fluka) was prepared into a series of standard solutions with a concentration of 10, 20, 40, 60, 80, and 100 mg / L with methanol, and the acetochlor standard with different concentrations was determined by high performance liquid chromatography (HPLC). The peak area of ​​the product was repeated 3 times. With the concentration of acetochlor as the abscissa and the peak area as the ordinate, draw the acetochlor standard curve.

[0066] The detection conditions are as follows:

[0067] Detection system: Agilent1100Series. Chromatographic column: C18Diamosil TM Reversed-phase column, 250mm×4.6mm, particle size 5μm. Chromatographic conditions: mobile phase: methanol: water = 80: 20 (v / v), water adjusted to pH = 3 with glacial acetic acid; dete...

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Abstract

The invention discloses a bacterium capable of degrading herbicide acetochlor and an application of the bacterium. The bacterium is mycobacterium sp. YCA11 and the preservation number of the bacterium is CGMCC (China General Microbiological Culture Collection Center) No. 7771. The mycobacterium sp. YCA11 with the preservation of CGMCC No.7771, disclosed by the invention, has the advantage that the degradation rate of the bacterium to 45mg/L acetochlor is up to 51.86% in 7 days in an inorganic salt culture medium, which indicates that the bacterium is capable of efficiently degrading the acetochlor and has a broad application prospect in the remediation of soil polluted by the acetochlor.

Description

technical field [0001] The invention relates to a bacterium for degrading the herbicide acetochlor and its application. Background technique [0002] Acetochlor, English general name acetochlor, chemical name 2-ethyl-6 methyl--N-ethoxymethyl-α-chloroacetanilide, structural formula as shown in formula 1: [0003] [0004] Formula 1. [0005] Acetochlor is a systemic amide herbicide and is one of the most widely used herbicides in agricultural production. It can be absorbed by young shoots and roots of weeds, inhibit the synthesis of weed protein, and cause the death of weeds. Used for corn, cotton, soybean, peanut, rapeseed, potato, sugarcane, sesame, sunflower, leguminous, cruciferous, solanaceae, compositae, umbelliferous and other vegetable fields and orchards to control annual grass weeds And some broad-leaved weeds, it has a good control effect on soybean dodder, but it is ineffective against perennial weeds, and the effective period in the soil can reach 2 months....

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/32A62D101/04A62D101/28
Inventor 孙建光顾金刚刘君胡海燕
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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