Liquid-phase chip kit for detection of acute coronary syndrome based on serum exosomes and preparation method

Abstract

The invention relates to a kit used for auxiliary judgment of risk stratification of patients with acute coronary syndrome based on detection of serum exosomes and a preparation method. The kit mainly comprises exosomes extraction reagents, relevant microballoons capturing antibodies (the above antibodies are one or some selected from CD11c, CD81, MHCII, CD86, CD106, CD54 and CD3), biotin labeled detection antibodies and streptavidin phycoerythrin. After being optimized, the diagnostic kit has advantages of high detection efficiency, less amount of required samples, high specificity, high sensitivity, fast and accurate detection and the like, so the risk stratification conditions of patients can be evaluated comprehensively, and relevant references are provided for clinical intervention.

Description

technical field

[0001] The present invention relates to the technical field of in vitro kits, in particular to an in vitro kit for risk stratification of patients with acute coronary syndrome, in particular to a liquid phase chip combined parallel detection method based on serum exosomes, namely exosomes and Kit and preparation method thereof. Background technique

[0002] Atherosclerosis (AS) vascular disease has become the "number one killer" that threatens the health of Chinese people. With the development of my country's economy, the incidence rate is increasing year by year. The annual medical expenses for such diseases in my country are as high as 100 billion Strengthening the prevention and treatment of AS vascular diseases and their complications has become the main task of the prevention and treatment of major diseases in our country.

[0003] Acute coronary syndrome (ACS) caused by AS vascular disease is the most common and serious clinical manifestation, and the r...