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Application of water-soluble carbon nano-grade material

A carbon nanomaterial, water-soluble technology, used in microorganisms, biochemical equipment and methods, tumors/cancer cells, etc., can solve the problems of uninhibited or eliminated cell growth and reproduction, and achieves easy popularization and application, low toxicity, and preparation. simple method effect

Inactive Publication Date: 2013-11-13
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, graphene quantum dots have been widely used in cell / in vivo imaging due to their excellent photostability and biocompatibility, but there have been no reports of functional carbon nanomaterials inhibiting or clearing mycoplasma, protecting cell growth and reproduction

Method used

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  • Application of water-soluble carbon nano-grade material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, functional carbon nanomaterial inhibits and removes mycoplasma, protects the effect of Hela cell from infringement

[0021] After graphene oxide (C2) is prepared according to the method of Chinese patent application 201110225484.6, the specific implementation steps of inhibiting mycoplasma infected cells are as follows:

[0022] First, the toxicity of C2 to Hela cells was determined. Take 2 mg of powdered C2 sample and disperse it in 4 mL of DMEM medium containing 10% calf serum and phenol red, filter it with a sterile filter membrane with a pore size of 0.2 um, and prepare a sterile C2 DMEM solution mother solution with a concentration of 0.5 mg / mL . Resuscitate Hela cells, adjust the cell density to 5×10 4 / mL, seed Hela cells into 96-well plates (7×6), at 37°C, 5% CO 2 Incubate for 24 hours under the same conditions, and then change to C2 DMEM solutions with different concentration gradients to continue culturing for 24 hours after the cells adhere t...

Embodiment 2

[0029] Example 2, the effect of functional carbon nanomaterials on inhibiting Ureaplasma urealyticum and protecting Hela cells in clinical urine samples

[0030] Specific implementation steps

[0031] First, a series of functional carbon nanomaterials are prepared. According to the literature Chem.Commun., 2009, 5118-5120, 2g glucose, 10mL polyethylene glycol (PEG-200) with a molecular weight of 200, 3mLH 2 O was microwaved for 10 min at a power of 1 kW, purified and dried to prepare carbon quantum dots (C1).

[0032] Graphene oxide (C2) was prepared according to the method of Chinese patent application 201110225484.6 embodiment 3;

[0033] Hollow fullerene C was prepared according to the method of Chinese patent application 201110225484.6 Example 2 60 Derivatives of (C3);

[0034] According to the literature (Science, 1996, 271 (5247), 317; Chem Ber, 1993, 126: 195), the C 70 Fullerene derivatives, C 70 [C(COOH) 2 ] 3 (abbreviated as C4);

[0035] According to litera...

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Abstract

The invention discloses an application of a water-soluble carbon nano-grade material. The invention relates to the application of the water-soluble carbon nano-grade material in preparing a product with mycoplasma infection inhibiting and / or mycoplasma removing functions. In the application, the water-soluble carbon nano-grade material has hydrophilic groups on the surface. The water-soluble carbon nano-grade material is at least one selected from fullerene, graphene quantum dot, carbon quantum dot, carbon nano-tube, carbon nano-fiber, graphene, graphene oxide, and carbon nano-horn aggregate. The carbon nano-grade material has good biocompatibility, and assists in effectively inhibiting and removing mycoplasma, such that cell activity can be protected to a certain extent.

Description

technical field [0001] The invention relates to the application of a water-soluble carbon nanometer material. Background technique [0002] Mycoplasma is the enemy of cell culture and the most common pollutant that is not easily detected and interferes with experimental results. It is also the nemesis of mycoplasma infection-related diseases in clinical medicine. For example, Mycoplasma pneumoniae can cause acute respiratory infection in children with pneumonia, that is, Mycoplasma pneumonia, and severe mycoplasma pneumonia can also lead to death; Ureaplasma urealyticum can cause inflammation of the patient's reproductive system, leading to female infertility. Mycoplasma infection of cells can significantly change cell growth characteristics, enzyme pathways, cell membrane composition, chromosome structure, and transfection efficiency. Unlike bacteria and fungi, mycoplasma infections cannot be detected by visual inspection. They are so small that they pass through most ste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/09
Inventor 舒春英江凤陈代钦李书沐王春儒万立骏
Owner INST OF CHEM CHINESE ACAD OF SCI
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