Tumor cell anoikis incubator

Abstract

The invention discloses a tumor cell anoikis incubator which comprises a 3D culture bracket filled with a cell culture medium and culture dishes arranged at two ends of the culture bracket; culture rooms filled with 3D materials are arranged in the culture dishes; the 3D culture bracket is communicated with the interiors of the culture dishes; an opening used for the cell culture medium to go in / out the culture rooms is formed in the culture room; the 3D culture bracket comprises two culture tanks oppositely arranged and at least one hollow spiral pipe for communicating the two culture tanks. The tumor cell anoikis incubator can simulate the in-vivo environment of a circulating tumor cell reliably, facilitate culturing the tumor cell and screening the anti-anoikis tumor cell, and provide an important experiment tool for studying tumor metastasis.

Description

technical field [0001] The invention belongs to the field of cell culture devices, and relates to a tumor cell anoikis culture device. Background technique [0002] Anoikis is the programmed cell death induced by the loss of contact between cells and extracellular matrix or adjacent cells, but some tumor cells can survive and become anti-anoikis cells, and some of them have strong vitality and high malignant degree. Cells can enter blood vessels to become circulating tumor cells, and these anti-anoikis cells can achieve distant metastasis of tumors through blood circulation. [0003] At present, the research model of tumor metastasis mechanism is limited to the metastasis model in mice, which is costly and takes a long time. No mature in vitro model has been published yet. To establish an in vitro model, the first step is to obtain anoikis-resistant cells in vitro. At present, the study of anti-anoikis models is still limited to agar plate culture, or the use of simple ex...

AI Technical Summary

Problems solved by technology

At present, the study of anti-anoikis models is still limited to agar plate culture, or the use of simple external force shaking suspension culture cells, so that the cells cannot adhere to the wall and grow, and cannot simulate the real environment of tumor metastasis in the human body. This method mainly has the following Questions: 1. The survival state of the anoikis-resistant cells cultured in this model cannot simulate the influence of the contact between cells and extracellular matrix, adjacent cells, local metabolic factors, and vascular microenvironmental factors; 2. The culture model obtained The cells are in the state of static culture or simple shaking, which cannot simulate the blood flow environment in which the cells survive, and cannot provide a model for the study of tumor metastasis.

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