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Method for ensiling enteromorpha and method for preparing enteromorpha crude polysaccharide

The technology of prolifera and seeds is applied in the field of in-depth development and utilization of prolifera resources, which can solve the problems of prolonging the storage period of fresh prolifera and the inability to store fresh prolifera for a long time. The effect of extending the shelf life

Active Publication Date: 2014-09-17
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is at first aimed at the problem that fresh Enteromorpha can't be stored for a long time, and a method for Enteromorpha green storage and a method for preparing Enteromorpha crude polysaccharides are provided. Using the microbial agent of the present invention for Enteromorpha green storage can Significantly extend the storage period of fresh Enteromorpha, and use the stored Enteromorpha to extract its crude polysaccharides, so that the resources of Enteromorpha can be fully utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the preparation method of the compound microbial bacterial agent that is used for Enteromorpha green storage

[0026] 1. The preparation process of Lactobacillus plantarum and Lactobacillus brevis powder is as follows:

[0027] Lactobacillus plantarum and Lactobacillus brevis bacteria powder can be commercially available Lactobacillus plantarum and Lactobacillus brevis (Changzhou Yijunjia Biotechnology Co., Ltd.), or screened from fresh yogurt or sauerkraut.

[0028] Slant medium (g / L): glucose 10, yeast 10, peptone 5, anhydrous sodium acetate 2, MgSO4?7H 2 O 0.02, MnSO4?H 2 O 0.001, NaCl 0.001, FeSO4?7H 2 O 0.001, agar 15, pH 6.5-7.0.

[0029] Seed medium (g / L): glucose 10, yeast 10, peptone 5, anhydrous sodium acetate 2, MgSO4?7H 2 O 0.02, MnSO4?H 2 O 0.001, NaCl 0.001, FeSO4?7H 2 O 0.001, pH 6.5-7.0.

[0030] Fermentation medium (g / L): glucose 40, yeast 20, peptone 10, anhydrous sodium acetate 2, MgSO4?7H 2 O 0.02, MnSO4?H 2 O 0.001, NaCl 0....

Embodiment 2

[0041] Embodiment 2: the preparation method of green storage enteromorpha

[0042] 200g of Lactobacillus plantarum, 100g of Lactobacillus brevis, 100g of Bacillus sime, and 100g of Bacillus pyrolyzed obtained by fermentation in Example 1 were mixed. Prepare Enteromorpha green storage with the following method with above-mentioned composite microorganism bacterium agent:

[0043] 1. Pre-construction preparation: Clean up the impurities in the green storage pond (4 m*3 m*1.5 m), dredge the drainage ditch, and ensure the smooth discharge of accumulated water in the pond.

[0044] 2. Raw material preparation: select fresh and non-rotten, 10 tons of Enteromorpha lumps processed with Enteromorpha extruding and dehydrating packaging equipment (patent number: 200910313001.0), and the water content of Enteromorpha treated by extrusion dehydrating and packaging equipment is about 50 -60%, pick up visible garbage and stones.

[0045] 3. Inoculation loading: stack the dehydrated Enterom...

Embodiment 3

[0048] Example 3: The preparation method of extracting Enteromorpha crude polysaccharides by using green storage Enteromorpha

[0049] Excavate 1600kg of green storage enteromorpha that embodiment 2 makes, water content is about 80%, drop in 3000 L reaction kettle, add the concentrated hydrochloric acid of 50kg, steam is warmed up to 120 ℃, after insulation 2 hours, open stirring and continue insulation 2 Hours, after the reaction is over, cool to room temperature, adjust the pH to neutral with 10mol / L potassium hydroxide solution, and obtain the aqueous solution of Enteromorpha crude polysaccharide by plate and frame filtration. Under the condition of air outlet temperature of 70-80°C, spray-dry to obtain Enteromorpha crude polysaccharide powder with water content less than 5%. The prepared enteromorpha polysaccharide has good water solubility, and can be diluted 800-1000 times and sprayed on the leaves of plants, which has obvious rooting and strong seedlings, and improves p...

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Abstract

The invention discloses a method for green storage of Enteromorpha and a method for preparing crude polysaccharides of Enteromorpha. The invention utilizes a compound microbial bacterial agent including Lactobacillus plantarum, Lactobacillus brevis, Bacillus sime, and Bacillus coldolyticus to carry out Enteromorpha green storage, Enteromorpha green storage, after the green storage method of the present invention, the firm cellulose structure becomes easy to decompose and utilize, and the acid hydrolysis process can not only reduce the amount of acid, but also improve the degradation efficiency and improve the hydrolyzate. Medium solid content, no need to be concentrated, can be directly spray-dried, reducing production costs. The prepared crude enteromorpha polysaccharide can improve the immunity and stress resistance of crops. The invention effectively solves the technical problem in green storage of enteromorpha and realizes long-term storage of fresh enteromorpha. Moreover, the method of the invention has simple operation, short process flow and low production cost, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of deep development and utilization of Enteromorpha resources, and in particular relates to a method for green storage of Enteromorpha and a method for preparing crude polysaccharides of Enteromorpha. Background technique [0002] Enteromorpha is a genus of Chlorophyta Ulvaceae. Enteromorpha itself is rich in carbohydrates, protein, crude fiber, fatty acids and vitamins, and also contains iodine, calcium, phosphorus, sodium, iron, potassium and other trace elements. Due to the specificity of the marine living environment, the active ingredients of Enteromorpha have unique physiological characteristics, so Enteromorpha is a seaweed resource with great development value. [0003] Due to the seasonality of Enteromorpha, when the weather is hot from June to August, the moisture content of the salvaged Enteromorpha is about 90%. It cannot be stored for a long time in high temperature weather, and it will rot and deteriora...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C08B37/00C12R1/07C12R1/25C12R1/24
Inventor 张术聪宋海妹单俊伟翟熙伦乔来艳
Owner 青岛海大生物集团股份有限公司
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