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Preparation method of fish fin tissues for extracting genomic DNA (deoxyribonucleic acid) of fish

A genome and fish fin technology is applied in the field of preparation of fish fin tissue for extracting genomic DNA of fish, which can solve the problems of high requirements for preservation conditions, unsuitable field sampling, inconvenient portability, etc., so as to reduce workload and ensure experiments. quality, the effect of reducing the space occupied

Inactive Publication Date: 2013-12-11
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problems that the current preparation method of fish fin tissue samples requires high storage conditions, is not suitable for field sampling and is inconvenient to carry, and provides a method for preparing fish fin tissue samples for the extraction of fish genome DNA

Method used

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  • Preparation method of fish fin tissues for extracting genomic DNA (deoxyribonucleic acid) of fish
  • Preparation method of fish fin tissues for extracting genomic DNA (deoxyribonucleic acid) of fish
  • Preparation method of fish fin tissues for extracting genomic DNA (deoxyribonucleic acid) of fish

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specific Embodiment approach 1

[0019] Specific embodiment one: present embodiment is used for the preparation method of the fin tissue of fish genome DNA extraction, carries out according to the following steps:

[0020] 1. Collect fresh fish fin tissue;

[0021] 2. Place the fin tissue flat on the filter paper and cover with another filter paper;

[0022] 3. Put the fin tissue covered with filter paper in a cool and ventilated place until the sample is completely dry;

[0023] 4. The samples are stored at room temperature and protected from moisture, and the preparation of fin tissue for fish genome DNA extraction is completed.

[0024] The method in this embodiment has the characteristics of simple operation process for the preparation of fin tissue used for fish genome DNA extraction, especially in the case of a large number of samples, it can reduce the workload, improve work efficiency, and at the same time ensure the quality of the experiment.

specific Embodiment approach 2

[0025] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 3, a silica gel desiccant can also be used to dry and dehydrate the fish fin tissue. Others are the same as in the first embodiment.

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Abstract

The invention provides a preparation method of fish fin tissues for extracting genomic DNA (deoxyribonucleic acid) of fish, relating to a preparation method of animal tissues for extracting the genomic DNA (deoxyribonucleic acid). The preparation method is used for solving the problems that an existing preparation method of a fish fin tissue sample of the fish requires high preservation conditions, and is unfit for field sampling and the carrying is inconvenient. The preparation method comprises the following steps: (1) collecting the fresh fish fin tissues; (2) flatly putting the fish fin tissues on filter paper, and covering the fish fin tissues by another piece of filter paper; (3) putting the fish fin tissues covered by the filter paper at a cool and ventilation place until the sample is totally dried; (4) carrying out damp-proof preservation on the sample under the room temperature. The preparation method has the advantages that the prepared fish fin tissue sample can be preserved for a long time under the normal temperature and does not need a low-temperature environment, so that the occupied space is reduced, and the preservation cost is lowered; the operation process is concise, the sample is convenient to carry, the workload can be reduced particularly when the quantity of the samples is relative large, the working efficiency is increased, and meanwhile, the experimental quality is guaranteed.

Description

technical field [0001] The invention relates to a method for preparing animal tissue for genomic DNA extraction. Background technique [0002] There are epithelial cells, bone cells and other cell tissues in fish fin tissue, which contain enough genomic DNA, which can be used for the extraction of fish genomic DNA. [0003] At present, in order to inhibit the activity of endogenous nucleases and prevent DNA from being degraded, after the fin tissue samples of fish are collected for genomic DNA extraction, they are usually fixed with 95% ethanol, at low temperature or directly in liquid nitrogen. save. Since both liquid nitrogen and high-concentration ethanol are contraband, the above-mentioned storage method requires high storage conditions and is not suitable for field operations, making it inconvenient to carry samples after collection. Contents of the invention [0004] The present invention aims to solve the problems that the current preparation method of fish fin ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00C12N15/10
Inventor 李超鲁翠云程磊郑先虎徐鹏孙效文
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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