Desmodium styracifolium total flavonoids and preparation method of Desmodium styracifolium total flavonoids and total polysaccharides

A technology of desmodium and total flavonoids, which is applied in the field of preparation of total flavonoids and total polysaccharides of desmodium, can solve the problems of unfavorable medicinal material utilization and achieve the effect of saving production costs

Inactive Publication Date: 2013-12-11
CHONGQING KERUI PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, there are currently reports on the extraction and purification processes of flavonoids and polysaccharides, but most of them are extracted separately, which is not conducive to the utilization of medicinal materials.

Method used

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  • Desmodium styracifolium total flavonoids and preparation method of Desmodium styracifolium total flavonoids and total polysaccharides
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  • Desmodium styracifolium total flavonoids and preparation method of Desmodium styracifolium total flavonoids and total polysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of the total flavonoids and total polysaccharides of embodiment 1 desmodium

[0028] (1) Take Desmodium, crush it, add 80% v / v ethanol 12 times the weight of Desmodium, reflux extraction twice, each time for 1.5h, filter, and use the dregs and alcohol extract for later use;

[0029] (2) Take the alcohol extract obtained in step (1), concentrate it to 0.5g raw medicinal materials / ml, apply NKA-9 macroporous adsorption resin, and use 3-4BV water (Molish reaction negative), 4BV10% ethanol, 4BV70% Ethanol, eluting, collecting 70% v / v ethanol eluate, recovering the solvent under reduced pressure, and drying to obtain the total flavonoids of Desmodium desmodium; wherein, the total content of quercetin and kaempferenin is 4.61%, and the content of flavonoids is calculated as rutin 51.39%, and the cream yield is 1.94%.

[0030] (3) Take the dregs obtained in step (1), add 10 times the amount of water to decoct twice, each time for 2 hours, combine the water ext...

Embodiment 2

[0031] The research of embodiment 2 total flavonoids preparation technology

[0032] 1 Materials and Instruments

[0033] 1.1 Experimental materials

[0034] Chrysanthemum chinensis (purchased from Sichuan Xinhehua Chinese Medicine Pieces Co., Ltd., batch number 1204046); rutin reference substance (batch number: MUST-11040302), quercetin reference substance (batch number: MUST-12020101), all purchased from Chengdu Mansi Special Biotechnology Co., Ltd., kaempferol reference substance (batch number: 0773-9910, purchased from China National Institute for the Control of Pharmaceutical and Biological Products); other reagents were of analytical grade.

[0035] Among them, the medicinal materials of Desmodium Desmodium were fully inspected according to the relevant regulations under the item "Desmodium Desmodium" in the 2010 edition of the Pharmacopoeia, and all of them met the regulations; the determination method of the total flavonoid content was self-designed, and the total fla...

Embodiment 3

[0156] The research of embodiment 3 total polysaccharide preparation technology

[0157] 1 Materials and Instruments

[0158] 1.1 Experimental materials

[0159] Desmodium herb (purchased from Sichuan Xinhehua Chinese Medicine Pieces Co., Ltd., batch number 1204046), glucose reference substance (MUST-11020603, purchased from Chengdu Master Biotechnology Co., Ltd.), and other reagents were of analytical grade.

[0160] Among them, the medicinal materials of Desmodium sativa were fully inspected in accordance with the relevant regulations under the item "Desmodium moss" in the 2010 edition of the Pharmacopoeia, and all of them met the regulations; the content of polysaccharides was self-inspected, and the content of polysaccharides of Desmodium sativa was preliminarily stipulated to be no less than 6.26%.

[0161] 1.2 Experimental Instruments

[0162] Ultraviolet spectrophotometer; ultrasonic processor (220V, 50Hz); Sartorius electronic analytical balance (accuracy of 1 / 100,00...

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Abstract

The invention provides Desmodium styracifolium total flavonoids. In the Desmodium styracifolium total flavonoids, the content of flavonoids (calculated as rutin) is 50-60 % (w/w), the total content of quercetin and kaempferol is 4-5 % (w/w). The invention further provides a method for extracting total flavonoids and total polysaccharides at the same time from Desmodium styracifolium. The invention further provides the use of Desmodium styracifolium total flavonoids and total polysaccharides. By the investigation of extraction and purification methods, the invention successfully extracts and separate Desmodium styracifolium total flavonoids and total polysaccharides at the same time from one and the same batch of pharmaceutical raw materials, so as to effectively utilize the pharmaceutical raw material resources and save the production cost, being more suitable for industrial production. Meanwhile, the invention further discloses that Desmodium styracifolium total flavonoids and total polysaccharides have good diuretic, calculus-removing and choleretic effects, which provides a guide for clinical medication.

Description

technical field [0001] The invention relates to total flavonoids of Desmodium, and also relates to a preparation method and application of total flavonoids and total polysaccharides of Desmodium, belonging to the field of medicine. Background technique [0002] Money grass is derived from Lysimachia christinae Hance. The whole plant, also known as Sichuan money grass, is a wild species, mainly produced in Sichuan, and also in Jiangxi, Hubei, Zhejiang, Jiangsu, Shanghai, Guangdong and other provinces. Distribution, the key points for identification are: herb, opposite leaves, kidney-shaped, entire margin, radial flowers, yellow, capsules, leaves in rosettes at the top of the stem, usually 2-8 opposite flowers at the top of the stem, Leaves and petals have black glandular strips; stem cross-section outline is round, there are many pigment cells in rhizome and leaf tissue, vascular bundles are ring-shaped, no non-glandular hairs, small glandular hairs, and secretory tract in st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53C08B37/00A61P1/16A61P13/04A61P13/12
Inventor 周在富刘睿斌刘俊敏苏其果吴华
Owner CHONGQING KERUI PHARMA GRP
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