Apocynum venetum eIF-5A gene and encoded protein thereof

Abstract

The invention discloses an apocynum venetum eIF-5A gene and an encoded protein thereof. A nucleotide sequence of the apocynum venetum eIF-5A gene is shown in SEQ ID No:1; an amino acid sequence of the encoded protein of the apocynum venetum eIF-5A gene is shown in SEQ ID No:2. The eIF-5A gene is cloned from apocynum venetum which has extremely strong stress resistance, medicinal value and economic value; a complementary deoxyribonucleic acid (cDNA) sequence of the gene is 909bp; an open reading frame is 480bp; 159 amino acids are encoded. The expression quantity of the AveIF-5A gene ascends in roots, stems and leaves under the stress conditions of salt and alkali, drought and low temperature; the saline-alkali tolerance of saccharomycetes can be improved by the intact open reading frame (ORF) and deleted sub-AveIF-5A86-156aa of the AveIF-5A; the plant resistance can be improved and the peroxidization of plant membrane lipid can be relieved under the stress conditions of salt and alkali according to over-expression of the AveIF-5A gene in a plant.

Description

technical field [0001] The invention relates to apocynum eIF-5A gene and its encoded protein. Background technique [0002] Desertification is a global problem, and the global desertification area is 40 million km 2 , and is still at 50,000-70,000 km per year 2 The rapid expansion of the world threatens nearly one-third of the world's land and the lives of more than one billion people. my country is one of the countries with the largest desertified land area in the world, with a desertified land area of ​​2.622 million km 2 , accounting for 27.3% of the total land area, the annual direct economic loss due to desertification reaches 54 billion yuan. Desertification is very serious in the western part of Heilongjiang Province, with an area of ​​54079.27km 2 , accounting for the province's total land area of ​​454,800 km 2 11.89%. Desertification has seriously affected the production of industry, agriculture, animal husbandry and people's lives. Agricultural ecology and ...

AI Technical Summary

Problems solved by technology

The study of eIF-5A in plants started relatively late. Although it has been reported that the introduction of eIF-5A into Arabidopsis, banana, and poplar improved the biomass and stress resistance of transgenic plants, the function and mechanism of the gene are not yet known. Not very clear, especially in adversity environment, the study of the biological function of the gene is even less

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