Micro-fluid cell processing chip and application method thereof

Abstract

The invention relates to a micro-fluid cell processing chip and an application method thereof. The micro-fluid cell processing chip consists of a bottom layer and a top layer, wherein the top layer is internally provided with an S-shaped pipeline and a cell operation chamber, the solution inflow inlet of the S-shaped pipeline is provided with two inflow channels, the inlet pipelines of the two inflow channels are respectively provided with a protective agent inlet and a buffer solution inlet, the solution outflow channel of the S-shaped pipeline is connected with the cell operation chamber which is provided with a solution outlet and a cell inlet and outlet channel which is provided with a torque control microvalve, two semi-partitions are respectively arranged at two sides of the cell operation chamber, so that cells can be restrained in the cell operation chamber and cannot be washed away. The cells are controlled to enter and exit the cell operation chamber through on and off of the torque control microvalve. According to the micro-fluid cell processing chip and the application method thereof, when the cryoprotectant for large volume of animal cells is loaded or removed, the concentration of the cryoprotectant can be changed continuously, so that the micro-fluid cell processing chip has the advantages of being simple and direct in operation, and having small damage to cells.

Description

technical field [0001] The invention relates to a microfluidic cell processing chip, in particular to a microfluidic microfluidic chip capable of continuously changing the concentration of the cryoprotectant in the process of loading and removing the cryoprotectant with little damage to cells and remarkable ease of operation Chips and methods of application thereof. Background technique [0002] Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in -196°C liquid nitrogen at low temperature can temporarily remove the cells from the growth state and preserve their cell characteristics, so that the cells can be revived for experiments when needed. Moreover, moderately preserving a certain amount of cells can prevent the cells from being lost due to contamination of the cells being cultured or other accidents, which plays a role in cell preservation. When cells are cryopreserved, add a protective agent to th...

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Problems solved by technology

The operation steps of this method are cumbersome, and it is required to accurately find the cells under the microscope in a short period of time and complete the transfer of the cells between the solutions, which is very easy to cause the loss of the cells and requires a high level of proficiency for the operator; And the number of step-by-step removals cannot be increased too much, so that the oocytes will still be subjected to several sudden volume changes due to sudden changes in the concentration of the protective agent, and the exposure time to high concentrations of cryoprotectant is long

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