Method for detecting chlorpromazine content of blood through high performance liquid chromatography

A high-performance liquid chromatography and chlorpromazine technology, which is applied in the field of high-performance liquid chromatography for detecting chlorpromazine content in blood, can solve problems such as inaccurate detection of chlorpromazine content, and achieve low detection costs, improved economic benefits, and improved operation. simple effect

Active Publication Date: 2013-12-18
上海兰卫医学检验所股份有限公司
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Problems solved by technology

[0006] In order to overcome the inaccurate shortcoming of existing chlorpromazine content detection, the invention provides a k

Method used

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  • Method for detecting chlorpromazine content of blood through high performance liquid chromatography
  • Method for detecting chlorpromazine content of blood through high performance liquid chromatography
  • Method for detecting chlorpromazine content of blood through high performance liquid chromatography

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment one: on the chromatogram with absorbance as the ordinate, the time is the peak when the retention time is 6.045 seconds and the chlorpromazine in the patient's blood when the retention time is 8.146 seconds. peak, according to Figure 6 According to the standard curve of chlorpromazine, the instrument automatically calculates that the content of chlorpromazine in the patient's blood is 85.30239ng / ml.

Embodiment 2

[0064] Embodiment two: on the chromatogram with absorbance as the ordinate, the time is the peak when the retention time is 6.067 seconds and the chlorpromazine in the patient's blood when the retention time is 8.254 seconds. peak, according to Figure 6 According to the standard curve of chlorpromazine, the instrument automatically calculates that the content of chlorpromazine in the patient's blood is 33.56788ng / ml.

Embodiment 3

[0065] Embodiment three: on the chromatogram with absorbance as the ordinate, the time is the peak when the retention time is 5.992 seconds and the chlorpromazine in the patient's blood when the retention time is 8.254 seconds. peak, according to Figure 6 According to the standard curve of chlorpromazine, the instrument automatically calculates that the content of chlorpromazine in the patient's blood is 13.37199ng / ml.

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Abstract

The invention relates to the technical field of biomedical treatment and provides a method for detecting the chlorpromazine content of blood through high performance liquid chromatography. The method comprises the steps of preparing 1mug/ml of a chlorpromazine standard solution, 1mug/ml of a diazepam internal standard solution, a blank sample, a normal human blood sample and a patient blood sample; putting into a chromatographic instrument to draw chromatograms, and judging whether the blood of a patient contains chlorpromazine according to the chromatograms; then drawing a chlorpromazine standard curve according to the chromatograms, comparing a chlorpromazine peak value in the chromatogram of the patient blood sample, a peak value of the diazepam internal standard solution and the chlorpromazine standard curve, and calculating the sodium valproate content of the blood of the patient. According to the method, the problems that the chromatograms contain multiple stray peaks, the sensitivity is low, the precision is low, the cost is high, the linear range of the drawn chlorpromazine standard curve is small, and peroxide often affects the stable extraction of the chlorpromazine are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a method for detecting the content of chlorpromazine in blood by high performance liquid chromatography. Background technique [0002] Chlorpromazine is a thiazide drug belonging to the dimethylamine group. It has obvious sedative effect and can produce strong anticholinergic effect and moderate extrapyramidal symptoms. It has strong antiemetic effect and can stop vomiting caused by various reasons. , is a representative drug of antipsychotics, and can be used as an antiemetic in small doses. [0003] Chlorpromazine is easily absorbed when taken orally, but the absorption is irregular, with large individual differences. The absorption rate is affected by the food in the stomach and anticholinergic drugs. The blood concentration reaches the peak. Chlorpromazine is highly fat-soluble and easily penetrates the blood-brain barrier. The concentration in the brain can reach 10 ti...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 冯娟甄燕
Owner 上海兰卫医学检验所股份有限公司
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