Macro fungus demethylation breading method and macro fungus demethylation breading device

Abstract

Disclosed are a macro fungus demethylation breading method and a macro fungus demethylation breading device. The device comprises a culture dish body and a culture dish cover, the culture dish body comprises a bottom disc and a side wall, and at least one annular partition is arranged in the culture dish body. The central area of the culture dish body is a pure culture medium area, and a culture medium A is added in the pure culture medium area; a culture medium A and an inhibitor B are added in mutation areas adjacent to the central area and continuous other mutation areas of the culture dish body; bacterial strains to be mutated are inoculated in the pure culture medium area, and are subjected to inverted culture under suitable-for-growth conditions; mycelia of the bacterial strains to be mutated in the central area grow into the adjacent mutation areas, and the inhibitor enters the growing mycelia to militate; mutant strains are acquired. The macro fungus demethylation breading method and the macro fungus demethylation breading device have the advantages that the device is simple in structure and convenient to use; the method is simple, mutation and detection can be performed synchronously, and mutation can be performed on macro fungi quickly and efficiently.

Description

technical field [0001] The invention relates to a large fungus demethylation breeding method and device. Background technique [0002] Macrofungi is a type of fungi that form large fruiting bodies in fungi, and generally refers to mushrooms or macrofungi in a broad sense. Macrofungi are an important group of fungi, and many species have high nutritional and medicinal value, and are currently the most promising category of fungi. my country has a history of macrofungi cultivation for thousands of years, and commercially cultivated macrofungi species are basically obtained through artificial breeding. For strains with clear biochemical pathways, it is easier to select strain improvement targets through metabolic engineering strategies; while for strains with unclear biochemical pathways, they can also be directly inherited through genome shuffling, ribosome engineering, and epigenetic modification. Selected to obtain the ideal phenotype. [0003] Direct blocking and high ex...

AI Technical Summary

Problems solved by technology

This method not only has a large workload and a long cycle, but the most important thing is that it cannot directly link mutagenesis and detection. Many mycelia that have changed cannot be detected because they are not coated on the petri dish, and the efficiency is relatively low.

Images