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Method for cultivating water lily aseptic seedling

A cultivation method and technology of sterile seedlings, which are applied in the field of seedling cultivation of water lilies and aquatic plants, can solve the problems of unstable chromosomal ploidy change of callus, low callus induction rate, and high pollution rate, and achieve the goal of overcoming emergence Irregularity and interference from external factors, thorough disinfection, and low seedling cost

Inactive Publication Date: 2013-12-25
ZHENJIANG SUIHAN AGRI
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Problems solved by technology

For the above-mentioned techniques, callus is selected as the explant to induce aseptic seedlings, and the chromosomal ploidy change of the callus is unstable; The rate of contamination is low, the rate of contamination is high, and the method of selecting callus as explants to cultivate sterile vaccines cannot be practically used

Method used

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Embodiment Construction

[0014] Below in conjunction with the cultivation method of the water lily variety " Peter " selfing seed aseptic seedling, the present invention will be further described.

[0015] This embodiment includes the following steps:

[0016] (1) Seed collection and treatment

[0017] Seed collection: Before the fruit is about to ripen and crack, pick the fruit and put it in a container with water. When the fruit matures and cracks to release the seeds, all stay in the container.

[0018] Seed washing: The seeds released from the fruit, with their membranous sheaths floating on the water surface, are mixed with sepals, petals and gums. Wrap these mixtures in a muslin cloth, squeeze out the gum and water, and rub them lightly with your hands. Rinse in water to remove residues and incompletely developed floating empty seeds, wash the seeds with clean water and store them. Seeds must be cleaned to thoroughly remove debris and immature seeds.

[0019] Seed storage: After washing t...

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Abstract

The invention discloses a method for cultivating water lily aseptic seedlings. The method for cultivating the water lily aseptic seedlings comprises the following steps: (1) collecting and treating seeds, namely selecting water lily seeds, washing by adopting sterile water, sterilizing for one minute by adopting 70% alcohol, then sterilizing by adopting 10% sodium hypochlorite, washing by adopting the sterile water under an aseptic condition, placing the water lily seeds into a sterile water bottle and carrying out germination accelerating; (2) cultivating and transplanting the young water lily seedlings, namely after third main leaves of the young water lily seedlings grow, respectively transplanting the young water lily seedlings into conical flasks filled with the sterile water and sterile fine pond sludge to be cultured, and when seventh or eighth main leave of the young water lily seedlings grow, transplanting the water lily seedlings into planting pots to be continuously cultured. The method for cultivating the water lily aseptic seedlings has the advantages that the massive seedlings can be directly propagated, the labour and the time are saved, and the cost is saved; the germination of the water lily seeds is uniform, the water lily seeds are thoroughly sterilized, the seedling culture cost is low, the emergence rate is up to more than 80%, the irregular emergence of the seedlings and interference caused by external factors during the conventional seeding of the water lily seeds are overcome, and the effect is obvious.

Description

technical field [0001] The invention relates to a method for raising seedlings of aquatic plants, in particular to a method for raising seedlings of water lilies, and belongs to the technical field of plant breeding. Background technique [0002] Nymphaea is a genus of Nymphaeaceae ( Nymphaea ) A general term for plants, water lilies are deeply loved by people for their rich colors, holy implication, and strong adsorption capacity of stems and leaves to nutrient and harmful substances in water, and are widely used in garden waterscapes. However, water lily seedlings are relatively tender and weak in the initial stage, and water lily seedlings are easily affected by external factors, and are also vulnerable to mosquitoes and chironomus larvae in the seedling raising process. Therefore, the cultivation of water lily seedlings must exclude the influence of these external factors, and the cultivation of sterile seedlings is undoubtedly the best way to solve this problem. At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 孙春青潘跃平戴忠良马志虎孙国胜
Owner ZHENJIANG SUIHAN AGRI
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