36results about How to "Guaranteed aseptic operation" patented technology

The invention relates to a novel normal-pressure room-temperature plasma induced mutation breeding device. The novel normal-pressure room-temperature plasma induced mutation breeding device comprises a sample processing system, a cooling system, a control system and a detection system; the sample processing system comprises a clean working chamber without bioactive contaminants; a step motor and an object stage are arranged in the clean working chamber; the object stage is arranged on the step motor; at least one sterilizing device mounting position is reserved in the inner cavity or on the wall of the clean working chamber; the detection system comprises a gas flow controller, a temperature sensor and a position sensor; and the control system comprises an operating panel and a controller, and the controller is used for controlling the step motor to realize rising and falling or horizontal rotation of the object stage so as to automatically process a plurality of samples. The novel normal-pressure room-temperature plasma induced mutation breeding device is capable of realizing the functions of continuous automatic processing, automatic sterilization, automatic monitoring and control on the samples, and also capable of completing the plasma induced mutation breeding in the normal pressure and room temperature environment at higher efficiency.

The present invention relates to a method capable of utilizing internal perfusion to induce bone marrow matrix stem cell to construct tissue engineering bone. Said method includes the following steps: (1), extraction, separation and culture of bone marrow matrix stem cell; (2), preparation of coral composite artificial bone; and (3), creation of perfusion system.

The present invention discloses a novel fermentation tank, which comprises a tank body, a membrane filter and a pipeline; wherein, the membrane filter is arranged inside the tank body; one end of the pipeline is connected with the membrane filter in the tank; the other end of the pipeline leads out of the tank or leads out of the tank through a hollow stirring shaft. The membrane filter can be arranged on the wall of the tank through the pipeline or on the hollow stirring shaft, and the included angle between the membrane filter and the longitudinal axis of the tank is between 0 degree and 90 degrees. In the process of bio-fermentation, the fermentation tank can carry out membrane separation, the positive pressure inside the fermentation tank or / and the negative pressure outside the fermentation tank ensure that liquid-phase material can permeate the membrane and pass through the pipeline to reach other devices outside the fermentation tank, and suspended cells and intercepted granules are intercepted in the fermentation tank to keep fermenting. The novel fermentation tank utilizes the characteristic of the combination of the fermentation tank and the membrane separation, to realize the coupling of cell culture and product abstraction. The fermentation tank does not affect the growth and metabolism of cells, the reaction effect is good, the biochemical reaction system is stable, moreover, the cost is low, investment is little, and the fermentation tank is applicable to biological reaction.

The invention belongs to the technical field of biotechnology, particularly relates to a biotechnological temperature-adjustable cell culture device, and aims to solve the problems that low-temperature adjustment can be adjusted, and culture is simple. The biotechnological temperature-adjustable cell culture device comprises a door, door hinges are hinged to positions, close to two ends, of the outer wall of one side of the door, the outer wall of one side of each door hinge is welded with the same culture box, fixing plates are welded at positions, close to two ends, of the outer walls of opposite sides of the door and the culture box, and the same spring is fixed to the outer wall of one side of each fixing plate through a bolt. Cells can be cryopreserved, cell dormancy time is prolonged, the cells can be taken out and recovered at any time when culture is needed, culture time is shortened, working efficiency is improved, the culture box can be constantly heated or cooled, the cell culture device is more suitable for cell reproduction and survival, a culture medium for a culture vessel and a cryopreservation tube can be cleaned before and after culture, the cells attached to thewalls can be washed down, and sterile operation is kept.

The invention relates to a T-type peritoneoscope sterile protective sleeve. The T-type peritoneoscope sterile protective sleeve comprises an imaging system sterile protective sleeve body and a cold light source sterile protective sleeve body which are arranged in a T type, wherein openings are formed in the two ends of the imaging system sterile protective sleeve body, one end of the cold light source sterile protective sleeve body, and the other end of the cold light source sterile protective sleeve body is integrally connected with the imaging system sterile protective sleeve body; the protective sleeve bodies are of an inner-outer two layer structure; a locking device winding the opening, arranged transversely, in one end of the imaging system sterile protective sleeve body, and a drawcord device used for folding the protective sleeve is concealed between the inner layer and the outer layer of each protective sleeve body is arranged at the other end of the imaging system sterile protective sleeve body and the opening end, arranged transversely, of the cold light source sterile protective sleeve body. The T-type peritoneoscope sterile protective sleeve is capable of reducing workload of medical workers and consequently shortening operation time and capable of the risk of operation infection.

The invention provides an ultrasonic cake cutting machine, comprising a frame, wherein an operation table is arranged in the middle of the frame; the operation table is provided with a movement receiving platform for bearing a cake; a liftable cutting device is arranged above the frame; the movement receiving platform comprises an X-axis transmission mechanism and a Y-axis transmission mechanism;the X-axis transmission mechanism and the Y-axis transmission mechanism are driven by an X-axis Y-axis driving portion, and the X-axis Y-axis driving portion comprises a synchronous belt for driving the X-axis transmission mechanism and the Y-axis transmission mechanism to move. According to the invention, the manual operation of cutting the cake is replaed with the machine with high efficiency, the cut cake is hygienic, the shape is regular, and both a circular cake and a square cake can be cut. Furthermore, the transmission form of the synchronous belt is adopted, and one synchronous belt completes driving in the X-axis and Y-axis directions, which reduces the pollution of a screw rod cylinder type driving device to a food production line, and ensures the whole-course sterilizing operation.

The invention discloses a mesenchymal stem cell preparation for treating knee osteoarthritis and a research method thereof. The research method comprises the following steps: preparing a platelet lysate, separating and culturing human umbilical cord MSCs, transplanting the human umbilical cord MSCs and evaluating the curative effect. The invention has the beneficial effects that the activity of prepared cells is ensured, the survival quantity of the cells can be used for experimental research, part of the cells are prevented from remaining in a culture bottle, the accuracy of cell counting is improved, the MSCs treatment is confirmed to be capable of obviously improving the pain of a patient suffering from knee osteoarthritis, and the effect is superior to that of a traditional hyaluronic acid treatment method; according to experimental data, the optimal treatment frequency can be selected, and a scientific basis is provided for treating knee osteoarthritis by using human umbilical cord mesenchymal stem cells in future.

An ovarian tissue section pushing device comprises a pushing core and an outer tube, wherein the outer tube coaxially sleeves the outside of the pushing core; the length of the pushing core is greater than or equal to the length of the outer tube; one end of the pushing core is fixedly connected with a handle; a projection is arranged at the other end of the pushing core and is positioned in the outer tube; and the projection is in sliding fit with the outer tube in a sealed manner. The ovarian tissue section pushing device is simple in structure and convenient to use, and transplanting time of an ovarian tissue section is greatly saved; and because the exposure time of the ovarian tissue section out of the body is short, recovery of an ovarian tissue and fertility of a postoperative patient is facilitated.

The invention discloses a surgical navigation interaction system and interaction method based on artificial intelligence and gesture control. The system comprises a camera module, a support module and a calculation module. The method comprises the following steps: 1, acquiring a gesture image through a camera module to form a gesture recognition data set; enabling each gesture to correspond to a gesture code through definition; 2, establishing a neural network model for gesture recognition through the gesture recognition data set; 3, deploying the neural network model in a calculation module; and 4, collecting a current gesture of a doctor through the camera module during an operation, inputting the current gesture into the calculation module, converting the current gesture into a gesture code corresponding to the gesture in the gesture image, and transmitting the gesture code to the operation navigation workstation to convert the gesture code into a corresponding operation code to realize the operation. The method is convenient to operate and high in gesture recognition accuracy; the artificial intelligence calculation speed is high, and intraoperative interaction is free of time delay; a background area can be eliminated, and misoperation caused by the environment is avoided; disinfection operation is not needed, and a non-contact interaction system can be realized.

The invention relates to an experimental apparatus for testing shear stress of cell fluid, which relates to the technical field of experimental devices for testing the shear stress of the cell fluid, and particularly relates to the experimental apparatus for testing shear stress of the cell fluid. The experimental apparatus comprises a principal computer (1), a pump controller (2), a peristaltic pump (3), a liquid storage tank (4), a support (5), a water outlet pipe (6), a water return pipe (7) and a fluid chamber (8), and is characterized in that the pump controller (2) is connected with the principal computer (1) and the peristaltic pump (3), the liquid storage tank (4) is installed on the support in a matching manner, a water returning opening is formed in the upper portion of the liquid storage tank (4), a water outlet is formed in the lower portion of the liquid storage tank (4), an inlet is formed in one end of the fluid chamber (8), an outlet is formed in the other end of the fluid cell (8), one end of the water outlet pipe (6) is connected with the water outlet of the liquid storage tank (4), and the other end is connected with the inlet of the fluid cell (8) in the matching manner by virtue of a pump head of the peristaltic pump (3).

The invention discloses a method for 3D reconstruction of a fracture reduction situation and gesture control image during an operation. The method comprises the following steps of: S1, performing CT scanning on a fracture part of a patient after reduction through a CT scanner, and importing the Diccom data of CT scanning into Mimics21.0 software; S2, converting the Diccom data into three-dimensional data by using the Mimics21.0 software; and S3, realizing intraoperative gesture operation and observing a fracture three-dimensional image by using a 3D gesture somatosensory controller. Accordingto the invention, the CT scanner is used for scanning the fracture part of a patient; unnecessary parts and artifacts of the scanned data are removed through a Mimics 21.0 software processor; the reconstructed three-dimensional image is displayed on a display screen; the 3D gesture somatosensory controller is used for carrying out rotating operation, so that whether the three-dimensional form of the fracture is good or not, whether the reduction is good or not, whether the articular surface is smooth or not and whether the screw is too long or protrudes into the joint or not can be conveniently observed in an operation, sterile operation can be guaranteed, a keyboard does not need to be touched, and the success rate of an orthopedic operation is effectively improved.

The invention discloses a bent plastic pipette head. The pipette head comprises a pipette head body and a filter element, wherein the pipette head body comprises a lower cavity, an upper barrel and aconnecting cavity connected with the lower cavity and the upper barrel, the lower cavity, the upper barrel and the connecting cavity are of an integrated structure, and the filter element is arrangedin an inner cavity of the lower cavity. The pipette head has the benefits as follows: compared with an original classical straight pipette head in each pipetting process, the operation action of the pipette head is more adapted to the artificial engineering principle, the operation process is easier and more convenient, the pipette head is particularly suitable for experiments requiring various long-term artificial pipetting operations, and the sterile effect in pipetting operation processes is guaranteed. The pipette head is bent, so that pressure gas flow is quickly downward under the regulation of the bent pipette head after leaving the oral liquid forwards and enters a mouth pipette under protection of a mask for various following precise pipetting operations.

The invention belongs to the technical field of medical apparatus and instruments, and particularly relates to a humidification bottle with a disposable inner cup, wherein the humidification bottle with the disposable inner cup is special for a medical float-type oxygen suction device. The humidification bottle with the disposable inner cup is composed of an outer bottle cover (1), an outer bottle (3), an inner cup cover (2) and an inner cup (4). The upper end threads (9) of the outer bottle cover (1) are arranged on the lower end portion of a float-type oxygen suction device valve body (8). Oxygen after being humidified is directly communicated with an oxygen outlet connector (7) of the float-type oxygen suction device valve body (8) without passing through a cavity between the inner cup (4) and the outer bottle (3). A groove (11) is arranged at the lower end of the outer bottle cover (1). A protruded block (12) is arranged at the upper end of the outer bottle (3). The inner cup (4) and the inner cup cover (2) are disposable products. The humidification bottle with the disposable inner cup is low in weight, simple and convenient to operate, quick to connect, low in humidification airflow noise, and capable of greatly reducing bacterium carrying rates and bacterium carrying quantity of the humidification bottle, and effectively avoiding cross infection inside a hospital during the use of the float-type oxygen suction device.