Mesenchymal stem cell preparation for treating knee osteoarthritis and research method thereof

A technology of knee osteoarthritis and stem cells, applied in the field of chemical medicine, can solve the problems of limited quantity and low immunogenicity

Pending Publication Date: 2021-06-01
HENAN YINFENG BIOENG CO LTD +1
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Problems solved by technology

[0003] Mesenchymal stem cells The advantages of mesenchymal stem cells in the treatment of osteoarthritis are that they have a wide range of tissue sources, easy expansion and differentiation, tissue-specific differentiated cells, anti-inflamma

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  • Mesenchymal stem cell preparation for treating knee osteoarthritis and research method thereof

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Embodiment Construction

[0016] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0017] see figure 1 , a mesenchymal stem cell preparation for treating knee osteoarthritis and a research method thereof, comprising the following steps:

[0018] Step A: Prepare the platelet lysate, take 300ml of venous blood from the patient, centrifuge to obtain the platelet lysate, store it at -80°C for later use;

[0019] Step B: Isolation and culture of human umbilical cord MSCs, rinse the umbilical cord with sterile saline in an ultra-clean bench, and ...

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Abstract

The invention discloses a mesenchymal stem cell preparation for treating knee osteoarthritis and a research method thereof. The research method comprises the following steps: preparing a platelet lysate, separating and culturing human umbilical cord MSCs, transplanting the human umbilical cord MSCs and evaluating the curative effect. The invention has the beneficial effects that the activity of prepared cells is ensured, the survival quantity of the cells can be used for experimental research, part of the cells are prevented from remaining in a culture bottle, the accuracy of cell counting is improved, the MSCs treatment is confirmed to be capable of obviously improving the pain of a patient suffering from knee osteoarthritis, and the effect is superior to that of a traditional hyaluronic acid treatment method; according to experimental data, the optimal treatment frequency can be selected, and a scientific basis is provided for treating knee osteoarthritis by using human umbilical cord mesenchymal stem cells in future.

Description

technical field [0001] The invention relates to a research method for a mesenchymal stem cell preparation, in particular to a mesenchymal stem cell preparation for treating knee osteoarthritis and a research method thereof, belonging to the technical field of chemical medicine. Background technique [0002] Osteoarthritis is a chronic bone and joint disease that seriously endangers human health. Cell therapy for osteoarthritis has developed rapidly in recent years and has attracted widespread attention. Umbilical cord-derived mesenchymal stem cells for osteoarthritis have both Mesenchymal stem cells are easy to expand and differentiate, have the advantages of anti-inflammation and recruitment, and have the advantages of cell rejuvenation, large amount of acquisition, no ethical issues, no pain in the process of sampling, high proliferation characteristics, pluripotent differentiation characteristics, and low immunogenicity , No tumorigenicity and other characteristics, it is...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K49/00A61P19/02C12N5/0775G16B40/00
CPCA61K35/28A61K49/0004A61P19/02C12N5/0668G16B40/00
Inventor 张雪梅王婷郭孟洋陈乐
Owner HENAN YINFENG BIOENG CO LTD
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