Preparation of novel TiO2/diatomite composite material, and method used for purifying phosvitin phosphopeptides with novel TiO2/diatomite composite material

A technology of phosvitin and composite materials, which is applied in the field of preparation of composite materials and separation and purification of biologically active substances, can solve the problems of short production cycle, numerous steps, complicated separation process, etc., achieve regular shape, high activity retention rate, The effect of high yield and purity

Active Publication Date: 2013-12-25
广东京豪生物制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using prepared TiO 2 /Diatomite composite material separation and purification of phosvitin phosphopeptide, easy operation, low cost, short production cycle, and

Method used

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  • Preparation of novel TiO2/diatomite composite material, and method used for purifying phosvitin phosphopeptides with novel TiO2/diatomite composite material
  • Preparation of novel TiO2/diatomite composite material, and method used for purifying phosvitin phosphopeptides with novel TiO2/diatomite composite material

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Weigh a certain amount of diatomite and disperse it in dilute hydrochloric acid with a solid-to-liquid ratio of 1:10, stir and react for 3 hours, then adjust the pH value to 5.0, then filter, wash, and calcinate to obtain a relatively pure carrier diatomite, wherein The concentration of dilute hydrochloric acid is 2M and the pH value is 4. Weigh a certain mass of purified diatomite, add hydrochloric acid with a solid-to-liquid ratio of 1:10 and a concentration of 2M, stir for 30 minutes, and then add a certain volume of TiCl 4 solution, continue to stir for 2h, then adjust the pH value of the reaction system to 4, and then react for 2h under stirring conditions to terminate the reaction. Filter and wash the material with deionized water until the filtrate is free of Cl ions, using a stirring rate of 500 rpm. The above materials were dried and calcined at 800°C for 5h to obtain TiO 2 / Diatomaceous earth composite material.

[0027] Use the acetone-ethanol extraction m...

Embodiment 2

[0030]Weigh a certain amount of diatomite and disperse it in dilute hydrochloric acid with a solid-to-liquid ratio of 1:10, stir and react for 3 hours, then adjust the pH value to 4.0, then filter, wash, and calcinate to obtain relatively pure carrier diatomite, wherein The concentration of dilute hydrochloric acid is 2M and the pH value is 4. Weigh a certain quality of purified diatomite, add hydrochloric acid with a solid-liquid ratio of 1:10 and a concentration of 0.1M, stir for 30 minutes, and then add a certain volume of TiCl 4 Solution, continue to stir for 2h, then adjust the pH value of the reaction system to 6, react under stirring conditions for 2h, terminate the reaction, filter, wash the material with deionized water until the filtrate does not contain Cl ions, and the stirring rate used is 400rpm. The above materials were dried and calcined at 500°C for 5h to obtain TiO 2 / Diatomaceous earth composite material.

[0031] Use the acetone-ethanol extraction method ...

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Abstract

The invention discloses preparation of a novel TiO2/diatomite composite material, and a method used for purifying phosvitin phosphopeptides with the novel TiO2/diatomite composite material, and belongs to the field of chemical composite material preparation and biologically-active substance purification technology. The invention provides an improved precipitation method used for preparation of supported nano-TiO2, and separation and purification of phosvitin phosphopeptides by using the supported composite material as an adsorption carrier. Diatomite material with a relatively large specific surface area is taken as a supported matrix, TiO2 is precipitated on to the surface of diatomite by chemical coprecipitation, and particle size and crystal form of the obtained TiO2 are controlled by controlling experiment conditions. The composite material prepared by the steps above possesses a relatively large specific surface area and a high coverage rate. The novel TiO2/diatomite composite material is used for separation and purification of phosvitin phosphopeptides, and is capable of increasing purification efficiency and adsorption capacity on phosvitin phosphopeptides. In addition, the novel TiO2/diatomite composite material can also be used for proteomics research of phosphoprotein, and identification of protein phosphorylation sites.

Description

technical field [0001] The invention relates to the technical field of composite material preparation and separation and purification of biologically active substances, in particular to a preparation of a composite material using metal oxides to load active carriers, and a method for separating and purifying phosvitin phosphopeptides. Background technique [0002] Phosvitin is the main phosphoprotein in egg yolk. Its primary structure contains 217 amino acid residues, 124 of which are serine residues, accounting for about 56% of the total amino acid residue content. Phosvitin contains a high net negative charge of about -179, which is the active carrier of cationic protein. Numerous studies have shown that phosvitin can interact with Ca 2+ , Mg 2+ , Mn 2+ ,Co 2+ ,Sr 2+ , Fe 2+ , Fe 3+ Wait for the combination. On the other hand, since phosvitin binds firmly with metal ions and is not easily hydrolyzed, its biological potency is low, which is not conducive to nutrient...

Claims

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Application Information

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IPC IPC(8): B01J20/14B01J20/30C12P21/06C07K1/14
Inventor 杨严俊苏宇杰张阳
Owner 广东京豪生物制药有限公司
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