Reagent kit for identifying duck hepatitis A virus infection and novel duck reovirus infection

A technology for duck hepatitis A virus and virus infection, which is applied in the detection/testing of microorganisms, resistance to vector-borne diseases, DNA/RNA fragments, etc., can solve the problems of high mortality, difficulty in distinguishing, and rapid onset, and achieve sensitivity High, low cost, good specific effect

Inactive Publication Date: 2013-12-25
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

[0004] Duck hepatitis A virus and new duck reovirus are very similar in terms of onset characteristics, clinical symptoms, and lesion characteristics. They have the common characteristics of mainly invading ducklings, rapid onset, high mortality, and liver hemorrhage as the main lesion. difficult to distinguish clinically
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  • Reagent kit for identifying duck hepatitis A virus infection and novel duck reovirus infection
  • Reagent kit for identifying duck hepatitis A virus infection and novel duck reovirus infection
  • Reagent kit for identifying duck hepatitis A virus infection and novel duck reovirus infection

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Embodiment Construction

[0050] 1. Design of Detection Primers

[0051] According to the conserved sequences of the 3D gene of DHAV and the 3S gene of NDRV registered in Genbank, a pair of primers were respectively designed by Primer5.0 software for the detection of duck hepatitis A virus and new duck reovirus;

[0052] The degenerate primers used for DHAV detection are:

[0053] Positive strand primer DHA1 (upstream primer): 5'-CTTTCYATCAATGACCARCG-3' (SEQ NO.1)

[0054] Negative strand primer DHA2 (downstream primer): 5'-GHADTTGMTCCATAACATCCTG-3' (SEQ NO.2)

[0055] (R=A or G; M=A or C; Y=C or T; H=A, T or C; D=G, A or T)

[0056] The size of the amplified fragment is 304bp.

[0057] The primers used for NDRV detection are:

[0058] Positive strand primer NDR1 (upstream primer): 5'-GTAAGCAACCTCAGGATATCG-3' (SEQ NO.3)

[0059] Negative strand primer NDR2 (downstream primer): 5'-TCATGTCGCGCGTCGTAT-3' (SEQ NO.4)

[0060] The size of the amplified fragment is 148bp.

[0061] 2. Optimization of re...

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Abstract

The invention discloses a reagent kit for identifying duck hepatitis A virus infection and novel duck reovirus infection. Specific primers (such as SEQ No. (sequence number) 1-4) of a duck hepatitis A virus and a novel duck reovirus are adopted to establish a double PCR (Polymerase Chain Reaction) detection method, and assembled into the reagent kit. The reagent kit can identify the duck hepatitis A virus and the novel duck reovirus in the same system and the mixed infection of the duck hepatitis A virus and the novel duck reovirus. The reagent kit has the characteristics of quickness, accuracy, high sensitivity, good specificity and the like, and can well solve the problem that the duck hepatitis A virus infection and the novel duck reovirus infection are difficult to distinguish clinically.

Description

technical field [0001] The invention relates to a kit for identifying duck hepatitis A virus and new duck reovirus infection, which is specially used for the differential diagnosis of duck hepatitis A virus and new duck reovirus infection, and belongs to the field of biotechnology. Background technique [0002] Duck viral hepatitis (Duck viral hepatitis) is also known as duck hepatitis, commonly known as "back neck disease". Ducklings under the age of 5 weeks are susceptible to the disease, and ducklings under the age of 3 weeks are the most susceptible and have a high mortality rate, some of which can reach more than 90%. The disease has a wide spread, high incidence and serious harm, and is one of the main diseases affecting the development of duck industry in my country. The disease is caused by Duck hepatitis A virus (DHAV), which is divided into three genotypes: A, B, and C. Currently, types A and C are mainly prevalent in my country. [0003] Novel duck reovirus (NDR...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCY02A50/30
Inventor 于可响李玉峰黄兵马秀丽史玉颖姜亦飞林树乾宋敏训李峰
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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