Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose

A technology of bacterial cellulose, dynamic culture, applied in specific-purpose bioreactors/fermenters, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low yield, complex process, etc., to achieve low energy consumption , The effect of simple process, strong structure and output controllability

Inactive Publication Date: 2014-01-01
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Existing methods have disadvantages such as low yield and complicated process. It is necessary to improve existing method

Method used

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  • Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose
  • Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose
  • Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A culture method in which a mechanical transmission device is used to drive the relative movement of the fermentation broth along the inner wall of the semi-permeable membrane, such as figure 1 shown.

[0032] 1. The height of the fermentation reactor is designed to be 100mm, and the diameter is 90mm. The side wall material of the main container is a 1.5mm thick silica gel plate. The blade length in the power unit is 20mm, and the central shaft is connected to a frequency conversion motor.

[0033] 2. Preparation of medium: add glucose 25g / L, yeast powder 7.5g / L, peptone 10g / L and disodium hydrogen phosphate 10g / L into a beaker filled with 1000ml ultrapure water in turn, and stir until it is completely dissolved; Glacial acetic acid adjusts the pH value of the system to between 4 and 5. After high-temperature sterilization at 115°C for 30 minutes, it was taken out and used as a medium for bacterial growth.

[0034] 3. Bacteria inoculation in the culture medium: cool t...

Embodiment 2

[0040] A culture method in which a magnetic stirring device is used to drive the relative movement of the fermentation broth along the inner wall of the semi-permeable membrane, such as figure 2 shown.

[0041] The preparation method step of present embodiment 2 is as follows:

[0042] 1. The height of the fermentation reactor is designed to be 100 mm, and the diameter is 90 mm. The side wall material of the main container is a silica gel plate with a thickness of 1.5 mm, and a magnet with a length of about 30 mm is selected.

[0043] 2. Preparation of culture medium: as in Example 1.

[0044] 3. Culture medium inoculated with bacteria: as in Example 1.

[0045] 4. Dynamic fermentation: Fill the entire reaction vessel with the culture solution inoculated with bacteria, and seal it with Vaseline after removing air bubbles. Adjust the rotating speed of the magnetic stirrer to about 100r / min, place the fermentation reactor in a well-ventilated constant temperature incubator a...

Embodiment 3

[0048] A culture method in which a magnetic stirring device is used to drive the relative movement of the fermentation broth along the inner wall of the semi-permeable membrane, such as figure 2 shown.

[0049] The preparation method steps of present embodiment 3 are as follows:

[0050] 1. The height of the fermentation reactor is designed to be 70mm and the diameter is 60mm. The side wall material of the main container is a silica gel plate with a thickness of 1.5mm, and a magnet with a length of about 30mm is selected.

[0051] 2. Preparation of culture medium: as in Example 1.

[0052] 3. Culture medium inoculated with bacteria: as in Example 1.

[0053] 4. Dynamic fermentation: Fill the entire reaction vessel with the culture solution inoculated with bacteria, and seal it with Vaseline after removing air bubbles. Adjust the rotating speed of the magnetic stirrer to about 300r / min, place the fermentation reactor in a well-ventilated constant temperature incubator at 30...

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Abstract

The invention discloses a fermentation reactor for dynamically culturing and preparing ordered bacteria cellulose. The fermentation reactor comprises a vessel body and a power device, wherein the side wall of the vessel body is made of an oxygen penetration material; the power device is used for driving a liquid in the vessel body to relatively rotate along the inner wall of the vessel body. The invention further provides a method for dynamically culturing and preparing the ordered bacteria cellulose, which comprises the following steps: preparing a culture medium; inoculating bacterium onto the culture medium; performing dynamic fermentation; fully filling a bacteria fermentation liquid in the vessel body in a sealing manner, controlling the rotation speed of the power device within a range of 100-500r/min, placing the fermentation reactor in a homothermal culture box at a temperature of 30 DEG C, culturing for 3-7 days, and obtaining cellulose gel; performing product purification. According to the invention, due to the adoption of the dynamic fermentation, the process is simple, and the prepared bacteria cellulose has a directionally arranged nano fiber structure, has higher porosity and transparency, and has the advantage that the controllability of the output and the structure of the product are strong during the fermentation.

Description

technical field [0001] The invention belongs to the field of preparation of bacterial cellulose materials, and relates to a device and method for dynamically cultivating and preparing ordered bacterial cellulose. Background technique [0002] Bacterial cellulose is very similar in structure to natural cellulose, both of which are high molecular compounds connected by glucose with β-1,4-glucosidic bonds. But bacterial cellulose has its unique characteristics: (1) high purity, high crystallinity, high degree of polymerization, large specific surface area and density; (2) ultra-fine three-dimensional network structure; (3) large elastic modulus, High tensile strength and tensile strength; (4) strong water holding capacity and good shape retention; (5) good biocompatibility and non-toxicity; (6) adjustable biosynthesis. Different culture methods and culture conditions can be used to obtain cellulose with different properties. [0003] Bacterial cellulose has the above excellen...

Claims

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Application Information

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IPC IPC(8): C12M1/02C12P19/04C12R1/01
CPCC12M23/22C12M27/10C12P19/04
Inventor 万怡灶胡达张琛罗红林王哲人
Owner TIANJIN UNIV
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