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Compound stabilizer for liquid ocean dextranase and application of compound stabilizer

A technology of dextranase and compound stabilizer, which is applied in the field of bioengineering, can solve the problems of being susceptible to changes in environmental factors and limiting the wide application of liquid dextranase preparations, and achieves obvious stabilizing effects, inhibited growth, and scientific compatibility Effect

Active Publication Date: 2014-01-08
上海丽恒口腔生物技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of industrial application of enzyme preparations, enzymes inevitably need to be transported and stored for a long time under certain conditions. The process is highly susceptible to environmental factors and microbial contamination, which severely limits the wide application of liquid dextran enzyme preparations

Method used

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  • Compound stabilizer for liquid ocean dextranase and application of compound stabilizer
  • Compound stabilizer for liquid ocean dextranase and application of compound stabilizer
  • Compound stabilizer for liquid ocean dextranase and application of compound stabilizer

Examples

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Effect test

Embodiment 1

[0020] Embodiment 1, a kind of liquid marine dextran enzyme compound stabilizer, this stabilizer is made by mixing and dissolving the raw materials of following weight ratio:

[0021] Sodium acetate 3; Sodium citrate 10;

[0022] Glycerin 6; Sodium Parabens 0.02.

Embodiment 2

[0023] Embodiment 2, a kind of compound stabilizer of liquid marine dextran enzyme, this stabilizer is made by mixing and dissolving the raw materials of following weight ratio:

[0024] Sodium acetate 24; Sodium citrate 26;

[0025] Glycerin 16; Sodium Parabens 0.07.

Embodiment 3

[0026] Embodiment 3, a kind of liquid marine dextran enzyme compound stabilizer, this stabilizer is made by mixing and dissolving the raw materials of following weight ratio:

[0027] Sodium acetate 10; Sodium citrate 20;

[0028] Glycerin 12; Sodium Parabens 0.04.

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Abstract

The invention provides a compound stabilizer for liquid ocean dextranase. The compound stabilizer for the liquid ocean dextranase comprises, by weight, 3-24 parts of sodium acetate, 10-26 parts of sodium citrate, 6-16 parts of glycerinum, and 0.02-0.07 part of p-hydroxybenzoic acid. After the stabilizer is added into the liquid dextranase and the liquid dextranase is kept for four months at the temperature of 4 DEG C, the relative enzyme activity of the dextranase can be kept above 85%. The compound stabilizer for the liquid dextranase is scientific in compatibility, obvious in stabilizing effect, low in cost, capable of maintaining high enzyme activity of the liquid dextranase, and strong in stability, thereby being an efficient stabilizer for the liquid dextranase and being significant for industrialization development of ocean dextranase liquid preparations.

Description

technical field [0001] The invention relates to a liquid dextran enzyme compound stabilizer, belonging to the field of bioengineering; the invention also relates to the application of the stabilizer. Background technique [0002] Dextranase (EC3.2.1.11) is a glycoside hydrolase that can hydrolyze α-1,6 glycosidic bonds in the dextran chain and chain end, so it is often used in the sugar industry and the production of plasma substitutes and prevention and treatment of dental plaque. Starting from the marine environment, a strain of Arthrobacter marine high-yield dextranase was screened. Compared with terrestrial enzymes, marine enzymes are more suitable for industrial applications due to their salt-, alkali-, and low-temperature tolerance. Industry, plasma substitutes, toothpaste, mouthwash production industrial dextranase. [0003] In the process of industrial application of enzyme preparations, enzymes inevitably need to be transported and stored for a long time under cer...

Claims

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Application Information

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IPC IPC(8): C12N9/96C12N9/44C12R1/06
CPCC12N9/2454C12N9/96C12Y302/01011
Inventor 王淑军吕明生王德龙王小贝焦豫良房耀维刘姝
Owner 上海丽恒口腔生物技术发展有限公司
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