Compound stabilizer for liquid ocean dextranase and application of compound stabilizer

A technology of dextranase and compound stabilizer, which is applied in the field of bioengineering, can solve the problems of being susceptible to changes in environmental factors and limiting the wide application of liquid dextranase preparations, and achieves obvious stabilizing effects, inhibited growth, and scientific compatibility Effect

Active Publication Date: 2014-01-08
上海丽恒口腔生物技术发展有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of industrial application of enzyme preparations, enzymes inevitably need to be transported and stored for a long time under certain conditions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound stabilizer for liquid ocean dextranase and application of compound stabilizer
  • Compound stabilizer for liquid ocean dextranase and application of compound stabilizer
  • Compound stabilizer for liquid ocean dextranase and application of compound stabilizer

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1, a liquid marine dextranase composite stabilizer, the stabilizer is made by mixing and dissolving raw materials with the following weight ratios:

[0021] Sodium acetate 3; Sodium citrate 10;

[0022] Glycerin 6; Sodium p-hydroxybenzoic acid complex ester 0.02.

Example Embodiment

[0023] Example 2, a liquid marine dextranase composite stabilizer, which is prepared by mixing and dissolving raw materials with the following weight ratios:

[0024] Sodium acetate 24; Sodium citrate 26;

[0025] Glycerin 16; Sodium p-hydroxybenzoic acid complex ester 0.07.

Example Embodiment

[0026] Example 3, a liquid marine dextranase composite stabilizer, the stabilizer is made by mixing and dissolving raw materials with the following weight ratios:

[0027] Sodium acetate 10; Sodium citrate 20;

[0028] Glycerol 12; Sodium p-hydroxybenzoic acid complex ester 0.04.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a compound stabilizer for liquid ocean dextranase. The compound stabilizer for the liquid ocean dextranase comprises, by weight, 3-24 parts of sodium acetate, 10-26 parts of sodium citrate, 6-16 parts of glycerinum, and 0.02-0.07 part of p-hydroxybenzoic acid. After the stabilizer is added into the liquid dextranase and the liquid dextranase is kept for four months at the temperature of 4 DEG C, the relative enzyme activity of the dextranase can be kept above 85%. The compound stabilizer for the liquid dextranase is scientific in compatibility, obvious in stabilizing effect, low in cost, capable of maintaining high enzyme activity of the liquid dextranase, and strong in stability, thereby being an efficient stabilizer for the liquid dextranase and being significant for industrialization development of ocean dextranase liquid preparations.

Description

technical field [0001] The invention relates to a liquid dextran enzyme compound stabilizer, belonging to the field of bioengineering; the invention also relates to the application of the stabilizer. Background technique [0002] Dextranase (EC3.2.1.11) is a glycoside hydrolase that can hydrolyze α-1,6 glycosidic bonds in the dextran chain and chain end, so it is often used in the sugar industry and the production of plasma substitutes and prevention and treatment of dental plaque. Starting from the marine environment, a strain of Arthrobacter marine high-yield dextranase was screened. Compared with terrestrial enzymes, marine enzymes are more suitable for industrial applications due to their salt-, alkali-, and low-temperature tolerance. Industry, plasma substitutes, toothpaste, mouthwash production industrial dextranase. [0003] In the process of industrial application of enzyme preparations, enzymes inevitably need to be transported and stored for a long time under cer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/96C12N9/44C12R1/06
CPCC12N9/2454C12N9/96C12Y302/01011
Inventor 王淑军吕明生王德龙王小贝焦豫良房耀维刘姝
Owner 上海丽恒口腔生物技术发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap