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Thermo-sensitive type injectable chitosan hydrogel product and applications thereof

A temperature-sensitive, chitosan technology, applied in the direction of immobilization on/in organic carriers, pharmaceutical formulations, pharmaceutical science, etc., can solve problems such as cross-linking agent toxicity, and achieve good biocompatibility.

Inactive Publication Date: 2014-01-22
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For the problems referred to above, the present invention proposes to crosslink the natural cationic polysaccharide chitosan with the natural cross-linking agent genipin that separates and obtains from geniposide, has invented a kind of thermosensitive injectable chitosan hydrogel, guarantees Under the premise of hydrogel strength, the problem of cross-linking agent toxicity is solved

Method used

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  • Thermo-sensitive type injectable chitosan hydrogel product and applications thereof

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Embodiment 1

[0026] 1. Dissolve chitosan with a molecular weight of 60kDa and a deacetylation degree of 50% in distilled water at 25.0g / L, and add Na at 12.5g / L 2 SO 4 , stirred for 30min, and got solution A for preservation;

[0027] 2. Dissolve genipin in physiological saline at 12.5g / L, stir for 2 hours to obtain solution B and store it;

[0028] 3. Before use, mix solution A and solution B at 4°C in a volume ratio of 4:1, stir for 10 minutes, heat up to 37°C, and form a gel after 50 minutes. The concentration of chitosan in the gel is 20.0g / L, Na 2 SO 4 Concentration is 10.0g / L, genipin concentration is 2.5g / L (attached figure 1 a, group A in b).

Embodiment 2

[0030] 1. Dissolve chitosan with molecular weight of 10kDa and deacetylation degree of 90% in 0.1mL / L hydrochloric acid solution at 30.0g / L, and add NaHCO at 6.0g / L 3 and Na 2 SO 4 , stirred for 30min, then adjusted the pH to 4.0 with 40.0g / L NaOH, and saved solution A;

[0031] 2. Dissolve genipin at 30.0g / L in distilled water and stir for 2 hours to obtain solution B for storage;

[0032] 3. Before use, mix solution A and solution B at a room temperature of 20°C in a volume ratio of 5:1, stir for 10 minutes, heat up to 30°C, and form a gel after 30 minutes. The concentration of chitosan in the gel is 25.0g / L. NaHCO 3 and Na 2 SO 4 Concentration is 5.0g / L, genipin concentration is 5.0g / L (attached figure 1 Group B in a, b).

Embodiment 3

[0034] 1. Dissolve chitosan with a molecular weight of 1000kDa and a degree of deacetylation of 80% in 10.0mL / L acetic acid solution at 20.0g / L, and then adjust the pH to 6.2 with 40.0g / L NaOH to obtain solution A for storage;

[0035] 2. Dissolve genipin at 1.0g / L in distilled water, stir for 2 hours, and add Na at 2.0g / L 3 PO 4 and Na 2 SO 3 , stirred for 30min, and got solution B for preservation;

[0036] 3. Before use, mix solution A and solution B at a room temperature of 4°C in a volume ratio of 1:1, stir for 10 minutes, heat up to 42°C, and form a gel after 20 minutes. The concentration of chitosan in the gel is 10.0g / L. Na 3 PO 4 and Na 2 SO 3Concentration is 1.0g / L, genipin concentration is 0.5g / L (attached figure 1 Group C in a, b).

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Abstract

The present invention relates to a thermosensitive injectable chitosan hydrogel. Genipin (a natural crosslinking agent) crosslinked natural cationic polysaccharide chitosan is used to prepare the product, which is a solution when the temperature is below 25°C and becomes a thermosensitive injectable hydrogel in an hour when the temperature is warmed up to 28-42 °C. The prepared hydrogel can be used as a drug carrier or a tissue engineering scaffold.

Description

technical field [0001] The invention relates to a temperature-sensitive hydrogel product, in particular to a genipin-crosslinked temperature-sensitive injectable chitosan gel product. Background technique [0002] Thermosensitive hydrogels are smart materials capable of solution-gel transition responses to temperature changes. By controlling its phase transition temperature to match the body temperature, a temperature-sensitive injectable hydrogel can be obtained: it is a flowable liquid at low temperature, and when injected into the target position of the human body, it will quickly solidify to form a hydrogel in a physiological environment . The temperature-sensitive properties of the thermosensitive hydrogel make it have the following characteristics: 1) The solution is easy to mix with drugs or cells at low temperature, and the gel can fix the drugs or cells when the temperature rises; 2) The solution mixed with drugs or cells can be transferred by injection, etc. 3) S...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08K5/1545C08J3/24C08J3/075A61K47/36A61K9/06A61L27/20A61L27/52C12N11/10
CPCA61L27/52C08L5/08A61L27/20A61K47/36A61K9/0024A61L27/3804A61L27/50A61L27/54A61L2400/06C08J3/075C08J2305/08
Inventor 马小军谢红国于炜婷添美
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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