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Method for detecting drug resistance of green peach aphid

A technology for drug resistance and green peach aphid, applied in the field of drug resistance detection of peach peach aphid, can solve the problems of no improvement, cumbersome operation, shortening the time for checking results, etc., and achieves the effects of intuitive and clear results, easy use, and overcoming the large number of samples.

Inactive Publication Date: 2014-01-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Toxicity determination is a common method for indoor understanding of insecticide resistance, but the traditional immersion method and drip method are cumbersome to perform, and several concentrations must be set and repeated to ensure the reliability of the results.
The glass tube drug film method is a new method for rapid detection of pest resistance. Due to its obvious effect, it shortens the time for checking results, but it is still the same as the traditional method in terms of concentration and repetition.

Method used

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  • Method for detecting drug resistance of green peach aphid
  • Method for detecting drug resistance of green peach aphid
  • Method for detecting drug resistance of green peach aphid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Obtaining of embodiment 1 diagnostic dosage

[0026] 1. Research object

[0027] The research object of the present invention is the mixed population of green peach aphids raised in the laboratory for multiple generations.

[0028] 2. Method

[0029] Toxicity determination of pirimicarb, bifenthrin, imidacloprid and acetamiprid was carried out by glass tube drug film method.

[0030] 2.1 Configuration and dilution of the mother liquor of the original drug

[0031] Dissolve the original drug in acetone, put it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C.

[0032] Before the toxicity test, the original drug mother solution was diluted to various concentrations with acetone and set aside.

[0033] 2.2 Preparation of glass tube drug film

[0034] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from the diluted drug solution an...

Embodiment 2

[0040] Example 2 Diagnostic Dosage Detection of Peach Aphid Resistance

[0041] 1. Research object

[0042] The object of this case study is the population to be tested in the field.

[0043] 2. Method

[0044] The test agents pirimicarb, bifenthrin, imidacloprid and acetamiprid were used to make glass tube drug films with the diagnostic dosage of the present invention, and the resistance level of green peach aphid was detected in the field.

[0045] 2.1 Configuration and dilution of the mother liquor of the original drug

[0046] The original drug is dissolved in acetone, and the diagnostic dose obtained by diluting the original drug mother liquor in Example 1 with acetone is set aside.

[0047] 2.2 Preparation of glass tube drug film

[0048] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from the diluted drug solution and add it to a glass tube with a diameter of 1.7cm and a height of 7...

Embodiment 3

[0052] Example 3 Determination of pesticide resistance of green peach aphid by the traditional glass tube drug film method.

[0053] 1. Research object

[0054] The object of this case study is the population to be tested in the field.

[0055] 2. Method

[0056] Toxicity determination of pirimicarb, bifenthrin, imidacloprid and acetamiprid was carried out by glass tube drug film method.

[0057] 2.1 Configuration and dilution of the mother liquor of the original drug

[0058] Dissolve the original drug in acetone, put it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C.

[0059] Before the toxicity test, the original drug mother solution was diluted to various concentrations with acetone and set aside.

[0060] 2.2 Preparation of glass tube drug film

[0061] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from the diluted drug solutio...

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Abstract

The invention provides a method for detecting the drug resistance of green peach aphid. Diagnosis glass tubes are produced under the diagnosis dosage of medicaments, and the resistance level of field green peach aphid population to the medicaments is rapidly detected, wherein the medicaments refer to pirimor, bifenthrin, imidacloprid or acetamiprid, the diagnosis dosage of pirimor is 10086.30ng / cm2, bifenthrin is 2159.00ng / cm2, imidacloprid is 47.86ng / cm2 and acetamiprid is 196.58ng / cm2. The method is simple and convenient in use, intuitive and clear in results, suitable for rapidly detecting the resistance level of field green peach aphid, overcomes the defects of large sample consumption amount, complex operation and time consumption of the traditional detection method, and can be used for rapidly detecting the resistance level of field green peach aphid population.

Description

technical field [0001] The present invention relates to a kind of measuring technology method that monitors the resistance of agricultural pests, specifically, detects the resistance of peach aphid to four kinds of insecticides (pirimicarb, bifenthrin, imidacloprid and pyridine) with the diagnostic dosage of the present invention. insecticide) resistance method. Background technique [0002] Green peach aphid is one of the most widely distributed and important pests in the world. It can feed on more than 400 kinds of plants in more than 50 families. It can not only directly suck the nutrients of plants, cause serious dehydration and malnutrition of plants, but also spread viruses. , cause greater indirect damage to plants, and cause huge economic losses to agricultural production. For a long time, the control of green peach aphid has been mainly based on chemical control. Due to the large, continuous and irrational use of chemical insecticides, peach aphids in many countrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N33/15
Inventor 高希武刘晓岚汤秋玲李永丹梁沛
Owner CHINA AGRI UNIV
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