Tissue-culture rapid multiplication method of nerviliae fordii corm

A technique for bulbs and bulbs of A. chinensis, which is applied in the field of tissue culture and rapid propagation of A. chinensis bulbs, can solve the problems of immaturity of seedling propagation technology, long cycle, depletion of wild resources, etc., so as to improve the reproduction speed and the multiplier of value-added, and shorten the seedlings. Effects of the reproductive cycle

Inactive Publication Date: 2014-01-29
YUNNAN DEHONG TROPICAL AGRI RES INST
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Problems solved by technology

[0002] Qingtiankui is a kind of precious traditional Chinese medicine, mainly distributed in Guangdong, Guangxi, Sichuan, Yunnan and other places. It is one of the "Southern medicines" in Guangdong real estate. , People randomly dig and dig, most of them are excavated and processed with leaves and bulbs, and wild resources are on

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[0010] Choose the corm that grows robustly and without pests to be the explant, and the specific method includes the following steps: (1) explant selection and disinfection: select the corm that grows robustly and without pests, soak it with washing powder earlier, and put it on the bulb with a toothbrush. The soil was washed clean, then soaked in 75% alcohol for 30s, placed in 0.1% mercuric chloride solution for disinfection for 10-15 minutes, and then rinsed with sterile water for 5-6 times; (2) Germination culture: inoculate the bulb until the germination On the medium, the germination medium is MS+BA2.0~4.0 mg / L+NAA0.5~1.0 mg / L+KT0.5~1.0 mg+LH (hydrolyzed milk protein) 1.0 g / L+sucrose 30 g / L liter, germination medium pH5.8~6.2, culture temperature 25~27°C, light intensity 1500~2000Lx, light 12 hours a day, culture time 30~45 days, induce rhizome; (3) rhizome proliferation culture : Inoculate the rhizomes on the proliferation medium, the proliferation medium is MS+BA2.0~4.0...

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Abstract

The invention provides a tissue-culture rapid multiplication method of nerviliae fordii corm and relates to the technical field of bioengineering. The tissue-culture rapid multiplication method is realized by the following scheme: the nerviliae fordii corm is used as an explant, sterilized and treated, germination culture is carried out onto the corm, and root stocks are obtained through culture; multiplication culture is carried out onto the root stocks to form a plurality of root stocks; and corm-induced culture is carried onto the root stocks to form a plurality of corm provenances. The method disclosed by the invention can be used for improving corm multiplication speed and proliferation rate of the nerviliae fordii and is beneficial to industrialized production of the nerviliae fordii corm.

Description

technical field [0001] The invention relates to a method for cultivating bulbs of medicinal blue skyflower, in particular to a method for tissue culture and rapid propagation of blue skyflower bulbs. Background technique [0002] Qingtiankui is a kind of precious traditional Chinese medicine, mainly distributed in Guangdong, Guangxi, Sichuan, Yunnan and other places. It is one of the "Southern medicines" in Guangdong real estate. , People randomly dig and dig, most of them are excavated and processed with leaves and bulbs, and wild resources are on the verge of exhaustion. Each corm can only develop into a complete plant, which not only has a low reproductive rate, but also has a long cycle. The second is to use tissue culture technology to propagate the blue skyflower seedlings, but the seedling propagation technology is still immature, and the reproduction coefficient Low. Contents of the invention [0003] In order to overcome the deficiencies in the existing blue sky...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李守岭白燕冰高燕
Owner YUNNAN DEHONG TROPICAL AGRI RES INST
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