Porcine circovirus II-type (PCV2) epitope peptide vaccine and preparation method thereof
A porcine circovirus and epitope peptide technology, which is applied to the preparation method of peptides, antiviral agents, virus antigen components, etc., can solve the problems of poor safety, large side effects, and high price, and achieve high reliability and strong specificity , good immunogenic effect
Active Publication Date: 2015-05-13
CHONGQING UNIV OF TECH +2
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Problems solved by technology
[0008] In view of the current domestic marketed whole-virus inactivated vaccines that have a certain protective effect, but have large side effects and poor safety, as well as the imported recombinant subunit vaccines and chimeric virus inactivated vaccines, which have good immunogenicity but are expensive Problem, the purpose of this invention is to provide a porcine circovirus type 2 (PCV2) epitope peptide vaccine that is cheap, safe, specific, easy to store and safe to use
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[0051] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.
[0052] 1. Prediction and analysis of porcine circovirus type 2 CAP protein B cell epitope:
[0053] PCV2 ORF2 is the gene encoding the main structural protein of the virus-Cap protein. This protein has good antigenicity and can cause high antibody levels in infected pigs, which can be used as a candidate vaccine antigen. The present invention selects PCV2b (90% domestic PMWS pig herds are all infected by PCV2b) CAP protein as a candidate antigen, and performs B cell epitope prediction analysis on its amino acid sequence.
[0054] The amino acid sequence of the PCV2 CAP protein is as follows (deduced from the genome sequence) with a total of 233 amino acid residues, retrieved from GenBank, and the sequence number is AAF35305.1.
[0055] 1 MTYPRRRYRR RRHRPRSHGLG QILRRRPWLV HPRHRVRWRR KNGIFNTRLS RTFGVTVKRT
[0056] 61 TVRTPSWAVD MMRFNINDFL PPGG...
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Abstract
The invention relates to a porcine circovirus type 2 epitope peptide vaccine and a preparation method thereof. The vaccine contains three B cell epitopes, with lysine as the core matrix to construct the same four-branched peptide as the epitope monomer, and a general T helper cell (Th) epitope in series with a molecular weight of 13kDa. The epitope peptide is named It is PCV CP98-156-228; the vaccine produces a strong immune response after immunizing mice and produces high-titer virus-neutralizing antibodies. The epitope peptide vaccine of the present invention has the advantages of low price, safety, strong specificity, easy storage and application, and will play an important role in preventing and controlling porcine circovirus disease. The method of the present invention prepares a porcine circovirus type 2 (PCV2 ) epitope peptide vaccine, the method has easy-to-obtain raw materials, low cost, easy control, good operability, and is suitable for popularization and application.
Description
technical field [0001] The invention belongs to the technical field of veterinary medicine, and in particular relates to a porcine circovirus type 2 (PCV2) epitope peptide vaccine and a preparation method thereof. Background technique [0002] 1. Porcine circovirus disease has become one of the three major diseases of pigs: [0003] Porcine circovirus type 2 (PCV2) is an important pathogen that causes post-weaning multisystemic wasting syndrome (PMWS), which was first confirmed in western Canada in 1997. The prevalence of this disease has been reported in most countries in North America, Europe and Asia. PCV2 is also associated with several other diseases, such as porcine dermatitis and nephrotic syndrome (PDNS), porcine respiratory disease syndrome (PRDC), porcine congenital tremor, sow reproductive failure, porcine hyperplastic and necrotizing pneumonia, etc. The diseases associated with PCV2 infection are collectively referred to as porcine circovirus associated disease...
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IPC IPC(8): A61K39/12C07K19/00C07K1/06C07K1/04A61P31/20
CPCY02P20/55
Inventor 吴胜昔吴玉章曾政熊仲良蔡家利梁望旺蔺露董春霞
Owner CHONGQING UNIV OF TECH