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De-browning method of browned calluses of taxus cuspidata

A callus, yew technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem that yew resources cannot meet the demand for paclitaxel production, and achieve sustainable callus growth, browning and other problems. High chemical removal rate and the effect of solving browning

Inactive Publication Date: 2014-02-12
DALIAN NATIONALITIES UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, with the continuous strengthening of protection measures for wild yew resources in the world, it is impossible to use wild resources to produce paclitaxel, and the amount of artificially planted yew resources at this stage is far from meeting the demand for paclitaxel production.

Method used

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  • De-browning method of browned calluses of taxus cuspidata
  • De-browning method of browned calluses of taxus cuspidata
  • De-browning method of browned calluses of taxus cuspidata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Disinfection of explants

[0030] Get the stem section of Taxus chinensis that was born in the same year, carry out aseptic treatment, and obtain the explant of the stem section of Taxus chinensis aseptic for subsequent use;

[0031] (2) Callus induction culture

[0032] Inoculate the sterilized Taxus explants obtained in step (1) on the induction medium, culture in dark at a controlled temperature of 18°C, and obtain callus after induction culture after 15 days;

[0033] The formula of described induction medium is specifically calculated according to per liter:

[0034] 3792mg KNO 3 , 15mg MnSO 4 ·H 2 O, 225mg CaCl 2 2H 2 O, 56 mg Na 2 EDTA·2H 2 O, 0.37mg CoCl 2 .6H 2 O, 0.39 mg CuSO 4 ·5H 2 O, 201mg (NH4) 2 SO 4 , 3mgZnSO 4 ·7H 2 O, 3.0mg NAA, 41.8mg FeSO 4 ·7H 2 O, 15mg VB1, 0.38mg NaMoO 4 .2H 2 O, 3.5mg IAA, 4.5mg H 3 BO 3 , 1.5mg Niacin, 220mg NaH 2 PO 4 .H 2 O, 1.5mg KI, 300mg Vc, 370mg MgSO 4 ·7H 2 O, 1.5mg VB 6 , 268.56mg inosito...

Embodiment 2

[0043] (1) Disinfection of explants

[0044] Get the stem section of Taxus chinensis that was born in the same year, carry out aseptic treatment, and obtain the explant of the stem section of Taxus chinensis aseptic for subsequent use;

[0045] (2) Callus induction culture

[0046] Inoculate the sterilized yew explants obtained in step (1) on the induction medium, culture in dark at a controlled temperature of 22°C, and obtain callus after induction culture after 15 days;

[0047] The formula of described induction medium is specifically calculated according to per liter:

[0048] 3792mg KNO 3 , 15mg MnSO 4 ·H 2 O, 225mg CaCl 2 2H 2 O, 56 mg Na 2 EDTA·2H 2 O, 0.37mg CoCl 2 .6H 2 O, 0.39 mg CuSO 4 ·5H 2 O, 201mg (NH4) 2 SO 4 , 3mgZnSO 4 ·7H 2 O, 3.0mg NAA, 41.8mg FeSO 4 ·7H 2 O, 15mg VB1, 0.38mg NaMoO 4 .2H 2 O, 3.5mg IAA, 4.5mg H 3 BO 3 , 1.5mg Niacin, 220mg NaH 2 PO 4 .H 2 O, 1.5mg KI, 300mg Vc, 370mg MgSO 4 ·7H 2 O, 1.5mg VB 6 , 268.56mg inositol,...

Embodiment 3

[0057] (1) Disinfection of explants

[0058] Get the stem section of Taxus chinensis that was born in the same year, carry out aseptic treatment, and obtain the explant of the stem section of Taxus chinensis aseptic for subsequent use;

[0059] (2) Callus induction culture

[0060] Inoculate the sterilized Taxus chinensis explants obtained in step (1) on the induction medium, culture in dark at a controlled temperature of 19°C, and obtain callus after induction culture after 15 days;

[0061] The formula of described induction medium is specifically calculated according to per liter:

[0062] 3792mg KNO 3 , 15mg MnSO 4 ·H 2 O, 225mg CaCl 2 2H 2 O, 56 mg Na 2 EDTA·2H 2 O, 0.37mg CoCl 2 .6H 2 O, 0.39mg CuSO 4 ·5H 2 O, 201mg (NH4) 2 SO 4 , 3mgZnSO 4 ·7H 2 O, 3.0mg NAA, 41.8mg FeSO 4 ·7H 2 O, 15mg VB1, 0.38mg NaMoO 4 .2H 2 O, 3.5mg IAA, 4.5mg H 3 BO 3 , 1.5mg Niacin, 220mg NaH 2 PO 4 .H 2 O, 1.5mg KI, 300mg Vc, 370mg MgSO 4 ·7H 2 O, 1.5mg VB 6 , 268.56m...

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Abstract

The invention discloses a de-browning method of browned calluses of taxus cuspidate. Namely, young immature stem sections or leaves of the current taxus cuspidate are used as explants to carry out induction on the calluses on a culture medium developed by an inventor, and then, the subculture is carried out on the calluses of the taxus cuspidate according to published documents, wherein a browning phenomenon easily exists in the calluses of the taxus chinensis in the subculture process. De-browned calluses of the taxus cuspidate in a good state can be re-induced on the browned calluses by carrying out culture on a subculture culture medium, developed by the inventor, of the calluses of the taxus cuspidate. The de-browning method disclosed by the invention not only can enable the browned calluses of the taxus cuspidate to be de-browned, but also can provide a technical support for producing taxol by applying a cell culture technology of the taxus cuspidate.

Description

technical field [0001] The invention relates to a method for debrowning the callus of the yew in the northeast that has been browned. Background technique [0002] Northeast yew, also known as yew. It is a tree plant of the Taxus family, a precious tree species left over from the Tertiary Period. This species is not only a timber plant, but also an important medicinal plant. Its stems, branches, leaves, and roots can be used as medicine, and its main components contain paclitaxel, taxine, and diterpenoids. It has anti-cancer function, and has the effect of inhibiting diabetes and treating heart disease. In addition, the seeds can also be used for oil extraction. Due to the slow growth of Taxus plants, the content of paclitaxel is low, accounting for only about one hundred thousandth of the dry mass of branches and leaves. Therefore, with the continuous strengthening of protection measures for wild yew resources in the world, it is impossible to use wild resources to prod...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李春斌岳玉莲刘宝全马洪丰于鹤王德萍
Owner DALIAN NATIONALITIES UNIVERSITY
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