Preparation method of stingray extract and its application in alcoholic liver protection drugs

Abstract

A preparation method of a stingray extract, which is characterized in that fresh stingrays are steamed and then the fish meat is removed, the cartilage is washed with clear water and minced, and NaOH solution is added according to the mass ratio of material to liquid at 1:1 to 1.5, and stirred for 4 hours. Then use HCl to adjust pH=6~7, filter, add NaOH to the filtrate to adjust pH=8.5~9.0, add composite protease to hydrolyze for 4 h according to the mass ratio of feed liquid and composite protease 1000:1. Heat the hydrolyzed solution at 80°C for 30 minutes, add HCl to adjust the pH=2, let it stand still, filter the upper layer solution with diatomaceous earth, add NaOH to adjust the pH=10.5, filter after hydrogen peroxide decolorization, add HCl to the filtrate to adjust the pH=7, and decompress the filtrate Concentrate to a small volume and dry the precipitate to obtain the stingray extract. The ray extract of the present invention can be used as a protective agent for alcoholic liver damage by enhancing the activity of antioxidant enzymes and enhancing the body's ability to remove peroxidation reaction products.

Description

technical field [0001] The invention relates to a preparation method of a stingray extract and its application in the preparation of a drug for protecting alcoholic liver damage. Background technique [0002] At present, alcoholism and addiction have become a global problem. Long-term heavy drinking can cause damage to multiple organs throughout the body, especially to the liver. Chronic alcoholism can lead to symptoms such as alcoholic fatty liver, alcoholic hepatitis, alcoholic cirrhosis, fibrosis, and in severe cases, canceration. At present, for the treatment of alcoholic liver poisoning, many methods include amino acid and glucose infusion, multivitamins, maintenance of water, electrolyte and acid-base balance, etc. In recent years, many researchers have tried to find a method that can effectively prevent and protect the liver from Medicines that lessen the damaging effects of alcohol on the liver. [0003] Stingrays, also known as devil rays, are the same kind of sha...

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Problems solved by technology

At present, there is no report on the preparation method of stingray extract and its application in the preparation of alcoholic liver injury protection drugs.