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Classification method of salmonella strains by using whole-cell fatty acid

A Salmonella and classification method technology, applied in the field of bioengineering, can solve the problems of insufficient classification and accuracy of germ classification, and achieve the effect of high utilization value, more classification, and fine classification.

Inactive Publication Date: 2014-02-19
中华人民共和国陕西出入境检验检疫局
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Problems solved by technology

[0004] However, as the types of food-borne pathogens become more and more complex, and the components of the pathogens become more and more similar, the classification of the pathogens in the existing classification methods is not precise enough and accurate enough.

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  • Classification method of salmonella strains by using whole-cell fatty acid
  • Classification method of salmonella strains by using whole-cell fatty acid
  • Classification method of salmonella strains by using whole-cell fatty acid

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Embodiment Construction

[0045] The present invention proposes a method for classifying Salmonella strains using whole-cell fatty acids. By adopting this method, it not only has the same detection ability as PFGE and serotype analysis, but also has the advantages of finer classification, faster classification and good economy. .

[0046] The concrete implementation process of the present invention is provided below:

[0047] The basis of adopting the present invention is to select multiple strains of Salmonella strains, and construct the fatty acid system dendrogram of Salmonella strains according to multiple strains of Salmonella strains;

[0048] In this experiment, the inventor selected a total of 42 strains of Salmonella, of which 4 strains came from human patients, 11 strains came from pork, 2 strains came from instant food, and 26 strains came from chicken. Thirty-nine strains of foodborne Salmonella were isolated in 2007 and 2009 from retail food in supermarkets and farmers' markets in Yanglin...

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Abstract

The invention relates to a classification method of salmonella strains by using whole-cell fatty acid, which belongs to the bioengineering technical field. The method comprises the following steps: respectively culturing a plurality of salmonella strains, extracting strain cells, acquiring types and content of aliphatic acid in strain cells, drafting an aliphatic acid system dendrogram of salmonella strains according to types and content of aliphatic acid of salmonella; employing the above method to obtain the types and content of aliphatic acid of salmonella strains to be measured, comparing the types and content with the aliphatic acid system dendrogram of salmonella strains, and then classifying the salmonella strains to be measured. The method provided in the invention has the advantages of precise classification for salmonella strains, faster classification speed and low application cost.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for classifying Salmonella strains by using whole-cell fatty acids. Background technique [0002] Salmonella is the most common foodborne pathogen and one of the main pathogens causing foodborne diseases in my country. It is estimated that nearly 22.2% of foodborne bacterial outbreaks in China are caused by Salmonella. Therefore, the typing of Salmonella, the main pathogen of food poisoning, and its correlation are studied to achieve accurate traceability, so as to quickly and effectively control the food poisoning caused by it. [0003] At present, there are many methods used to distinguish Salmonella, mainly including antibiotic susceptibility test, phage typing, serotype classification, multilocus enzyme electrophoresis (MLEE), pulsed field gel electrophoresis (PFGE), amplified fragment length polymorphism sex (AFLP) and multilocus sequence typing...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/42
Inventor 张鹏飞付骋宇蓝海啸刘德昊王新
Owner 中华人民共和国陕西出入境检验检疫局
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