Artificially modified high-activity Mariner-Like transposase

Abstract

The invention discloses artificially modified high-activity Mariner-Like transposase. Active transposase cloned from moso bamboo and plant MLE (Mariner-Like Elements) transposase with higher activity obtained after molecular optimization of the active transposase lay a foundation for developing genetic labels by means of the MLE transposase and provide a powerful guarantee for large-scale separation and marking of gene functions in the gene function.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to several highly active Mariner-Like transposases. Background technique [0002] A transposon is a DNA sequence that can be transferred from one site to another on the genome. Since the American geneticist McClintock first discovered transposons (Ac / Ds) in maize in the 1940s, scientists have discovered many types of transposons, which are widely found in bacteria, yeast and higher animals and plants. With the continuous deepening of people's understanding of the structure and function of transposons at the molecular level, some transposons have been transformed into gene tags for gene analysis, and have gradually become one of the important means of large-scale isolation of plant genes. [0003] Mariner-Like Transposons (Mariner-Like Elements, MLE) are an important family of transposons, which were first discovered when studying an unstable mutation in the wh...

AI Technical Summary

Problems solved by technology

However, the MLE transposase isolated in nature has accumulated more or less mutations due to the "vertical inactivation" effect during the evolution process, and has partially or completely lost the ability to catalyze transposition, becoming a low-activity or inactive transposase. It affects the application of MLE transposons, so it is very important to artificially construct highly active transposases

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