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Method of predicting reproductive capacity of field brown planthoppers through single nucleotide polymorphic mark

A single nucleotide polymorphism, brown planthopper technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as predicting the fecundity of insect populations, and achieve the effect of saving manpower and material resources and shortening the experimental period.

Inactive Publication Date: 2014-02-26
SUN YAT SEN UNIV
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  • Claims
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Problems solved by technology

However, there is no research report on the prediction of insect population fecundity by SNP

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  • Method of predicting reproductive capacity of field brown planthoppers through single nucleotide polymorphic mark
  • Method of predicting reproductive capacity of field brown planthoppers through single nucleotide polymorphic mark

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Embodiment Construction

[0022] The method of the present invention will be further described in detail below in conjunction with specific examples, so as to better understand the present invention.

[0023] 1. Collect female brown planthoppers and extract single genome DNA.

[0024] More than 30 female brown planthoppers (just migrated to the field) were collected from the field, and the genomic DNA of a single head was extracted. Take the Genomic DNA Isolation Kit (OMEGA, USA) kit as an example for extracting DNA. The extraction steps are as follows:

[0025] 1. Take the worms and grind them thoroughly in liquid nitrogen, +500ul Buffer GTC / 2-Me;

[0026] 2. Vigorously vortex for 15s (the more fully the cells are lysed, the more nucleic acids will be released), let stand at room temperature for 2-3min, and the longest can be extended to 5min;

[0027] 3. Centrifuge at 13,000xg for 5 minutes;

[0028] 4. Transfer the supernatant to In DNA Column, centrifuge at 13,000xg for 1min;

[0029] 5. Put...

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Abstract

The invention provides a method of predicting reproductive capacity of field brown planthoppers through a single nucleotide polymorphic mark. The method comprises the following steps: (1) collecting females of brown planthoppers, and extracting the genomic DNA (Deoxyribonucleic Acid) of the single female; (2) by taking the DNA as a template, respectively amplifying fragments of three SNP (Single Nucleotide Polymorphism) containing ACE-862, VGR-816 and VG3087 by a primer; (3) sequencing the PCR (Polymerase Chain Reaction) products to obtain the genotypes of the three SNP of each female; (4) introducing the SNP information obtain into a predicting model to calculate the reproductive capacity of the single brown planthopper. The reproductive capacity of the field brown planthopper population can be quickly and accurately predicted by means of the method provided by the invention, thereby saving the manpower and material resources and shortening the experimental period.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for predicting the fecundity of brown planthoppers in the field. Background technique [0002] Rice is the most important food crop in my country and even in the world, and its output is directly related to food security. Brown planthopper (Nilaparvata lugens) is an important pest of rice. It has strong adaptability and great variation in harmfulness. Its long-winged type has long-distance migratory ability. loss, food security is also threatened (Cheng Xianian et al., 2003). The prediction of the population of brown planthopper includes long-term prediction and short-term prediction. The long-term and medium-term predictions are mainly based on field insect data over the years, Markov chain theory and meteorological data. The short-term prediction of the population is mainly based on the amount of immigrant insects, survival rate and multiplication multiple. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张文庆孙仲享翟一凡张鉴清康奎邱洁琪沈嘉炜
Owner SUN YAT SEN UNIV