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Two-phase oil-emulsion adjuvant for veterinary vaccine and preparation method thereof

A dual-phase oil and emulsion adjuvant technology, which is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, emulsion delivery, etc., can solve the problems of technical difficulties in preparation, poor vaccine stability, animal side reactions, etc., and achieve process simplification , excellent stability, good stability

Active Publication Date: 2016-05-04
SICHUAN BAINUOJI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Water-in-oil (W / O) vaccines can obtain a higher level of immune response, and the immune protection period is longer, but there are also major defects: high viscosity, not conducive to injection, and often lead to severe animal side effects , such as body temperature and height of immunized animals, not eating, edema, induration, necrosis at the injection site, etc.
However, the preparation technology is difficult and the stability of the vaccine is poor

Method used

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  • Two-phase oil-emulsion adjuvant for veterinary vaccine and preparation method thereof
  • Two-phase oil-emulsion adjuvant for veterinary vaccine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A biphasic oil-emulsion adjuvant for veterinary vaccines, comprising mineral oil, Tween 80, Span 80, propylene glycol, polyvinyl acetate and glycerol monooleate, the weight percentages of each substance are respectively: mineral oil is 88.5 %, Tween 80 is 2.5%, Span 80 is 2.5%, propylene glycol is 2.5%, polyvinyl acetate is 2%, and glyceryl monooleate is 2%. Described mineral oil is the white oil of food or pharmaceutical grade.

[0029] The preparation method of biphasic oil-emulsion adjuvant for veterinary vaccine described in the present embodiment 1 comprises the following steps:

[0030] Step 1, get the mineral oil that is 88.5% by weight, the Tween 80 of 2.5%, the Span 80 of 2.5%, the propylene glycol of 2.5%, the polyvinyl acetate of 2%, the glyceryl monooleate of 2%, weigh Reserve after measurement;

[0031] Step 2, adding mineral oil into the reaction tank for stirring, the stirring speed is controlled at 86-96 rpm, after stirring for 28-40 minutes, the tempe...

Embodiment 2

[0037] A biphasic oil-emulsion adjuvant for veterinary vaccines, comprising mineral oil, Tween 80, Span 80, propylene glycol, polyvinyl acetate and glycerol monooleate, the weight percentages of each substance are respectively: mineral oil is 84.5 %, Tween 80 is 3.5%, Span 80 is 3.5%, propylene glycol is 3.5%, polyvinyl acetate is 2.5%, and glyceryl monooleate is 2.5%. Described mineral oil is the white oil of food or pharmaceutical grade.

[0038] The preparation method of biphasic oil-emulsion adjuvant for veterinary vaccine described in the present embodiment 2 comprises the following steps:

[0039] Step 1, get the mineral oil that is 84.5% by weight, the Tween 80 of 3.5%, the Span 80 of 3.5%, the propylene glycol of 3.5%, the polyvinyl acetate of 2.5%, the glyceryl monooleate of 2.5%, weigh Reserve after measurement;

[0040] Step 2, adding mineral oil into the reaction tank for stirring, the stirring speed is controlled at 86-96 rpm, after stirring for 28-40 minutes, t...

Embodiment 3

[0046] A two-phase oil-emulsion adjuvant for veterinary vaccines, comprising mineral oil, Tween 80, Span 80, propylene glycol, polyvinyl acetate and glycerol monooleate. The weight percentages of each substance are respectively: 86.5% for mineral oil, 3.1% for Tween 80, 3.1% for Span 80, 3.1% for propylene glycol, 2.1% for polyvinyl acetate, and 2.1% for glycerol monooleate. Described mineral oil is the white oil of food or pharmaceutical grade.

[0047] The preparation method of biphasic oil-emulsion adjuvant for veterinary vaccine described in the present embodiment 3 comprises the following steps:

[0048] Step 1, get the mineral oil that is 86.5% by weight, the Tween 80 of 3.1%, the Span 80 of 3.1%, the propylene glycol of 3.1%, the polyvinyl acetate of 2.1%, the glyceryl monooleate of 2.1%, weigh Reserve after measurement;

[0049] Step 2, adding mineral oil into the reaction tank for stirring, the stirring speed is controlled at 86-96 rpm, after stirring for 28-40 minute...

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Abstract

The invention discloses a two-phase oil emulsion adjuvant for veterinary vaccines. The two-phase oil emulsion adjuvant for veterinary vaccines comprises the following compositions in percentage by weight: 84.5%-88.5% of mineral oil, 2.5%-3.5% of tween 80, 2.5%-3.5% of span 80, 2.5%-3.5% of propylene glycol, 2%-2.5% of polyvinyl alcohol, and 2%-2.5% of monoolein. The invention further discloses a production method for the two-phase oil emulsion adjuvant for veterinary vaccines. The two-phase oil emulsion adjuvant for veterinary vaccines is good in stability and less in side effect, can provide long and stable immune protection, vaccines can be prepared through the adjuvant by adopting one-step emulsion process, and the technology is simplified, and suitable for market promotion and application.

Description

technical field [0001] The invention relates to a two-phase oil-emulsion adjuvant for veterinary vaccines and a preparation method thereof. Background technique [0002] The oil-emulsion adjuvant for veterinary vaccines is a substance that can be used before or at the same time as the antigen, which can enhance the body's non-specific immunity and the immunogenicity of the antigen, but does not have antigenic properties itself. Oil-emulsion adjuvant vaccine is a vaccine that uses mineral oil and emulsifiers as immune enhancers. It is one of the most widely produced types in the vaccine market at home and abroad. Oil-emulsion adjuvant vaccines can be divided into three dosage forms: water-in-oil ( W / O) type, oil-in-water (O / W) type and water-in-oil-in-water (W / O / W) type. [0003] Water-in-oil (W / O) vaccines can obtain a higher level of immune response, and the immune protection period is longer, but there are also major defects: high viscosity, not conducive to injection, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/113A61K39/39A61P37/04A61K39/135
Inventor 吴福文
Owner SICHUAN BAINUOJI TECH CO LTD