The invention discloses anti-microbial mildew-proof plastic wrap and a making method thereof. The anti-microbial mildew-proof plastic wrap is prepared from, by weight, polyvinyl alcohol, phthalic acid, triethyl citrate, polyethylene, chitosan, plasticizer, natural antibacterial agent, artemisia desertorum seed oil, endoconcha sepiae and glycerol monooleate. The natural antibacterial agent is prepared from, by weight, loess powder, folium eriobotryae extract, walnut leaf extract and Chinese tallow leaf extract. The anti-microbial mildew-proof plastic wrap is free of pollution, relatively environmentally friendly to food, high in plasticity and easy to prepare, has excellent mechanical strength and anti-aging performance and high stretchability and tearing property, has the advantages of being wide in antibacterial spectrum, even in distribution, efficient, durable and safe, and can effectively inhibit growth and reproduction of various malignant bacteria, fungi and mildew. Meanwhile, the anti-microbial mildew-proof plastic wrap is high in barrier property, air permeability, moisture penetrability and gas replacement capacity in actual application, and can purify the environment, eliminate odor and achieve self-cleaning and mildew resistance.