Enrichment applications for epidermal cells

Abstract

The invention relates to an enrichment application of epidermal cells, belonging to the technical field of biological cells. Including pretreatment, digestion and spraying, take the epidermis, make several pieces of epidermis, put the epidermis in a digestion container, add digestive enzymes, and carry out dynamic digestion to obtain a cell suspension; combine the digested cell suspension with protein gel Mix, mix well and spray on the wound. Applying the invention to wound repair has the advantages of high culture efficiency, convenient operation, high utilization rate of skin source, and the like.

Description

technical field [0001] The invention relates to the enrichment and application of epidermal cells, belonging to the technical field of biological cells. Background technique [0002] The incidence of burns in my country is 2 to 10‰ of the total population, and nearly ten million people are burned every year, and hundreds of thousands of people need to be hospitalized. Moreover, the death rate of severe burns is very high. After burns are cured, a large number of scars are often left behind, which will have a negative impact on the patient's appearance, body shape, function, and psychology. Among them, severe skin loss caused by extensive burns is an important factor leading to the death of patients, and the key to solving the problem is the skin source. [0003] In order to overcome the problem of the skin source, the method of closing the burn wound as early as possible is usually adopted. The early closure of the burn wound can not only save the life of the patient, but a...

AI Technical Summary

Problems solved by technology

[0004] (1) The micro-skin technology needs to remove the healthy skin source from the unburned part of the patient's body first, and then use physical methods to disperse the limited autologous skin source into as small particles as possible, and then replant it on the wound surface of the patient. This technique can enlarge the skin graft by 10 to 20 times, but this method uses physical methods to crush the skin graft, and then replants it on the wound surface. The adhesion rate of the grafted skin particles is not high, so the effect is difficult to guarantee;

[0005] (2) The method of culturing epidermal cells requires a long waiting time, and the long-term efficacy is not good;

[0006] (3) Recell technology is to digest the basal epidermal cells by trypsin under static conditions, and then spray the liquid epidermal cells suspended in Ringer's solution on the wound surface by spraying method. Using this technology, the skin graft can be enlarged by 40 ~60 times, but the disadvantage is that this method needs to consume a large amount of high-concentration digestive enzymes, and physical methods such as scraping will be used to sample during the process, and physical methods such as scraping will vary depending on the technical proficiency of the operator. There will be some damage, and the cell viability obtained through Recell is only about 70%. Therefore, the actual amount of epidermal stem cells obtained is difficult to confirm. The cells are evenly distributed on the wound surface, so the healing of the burn wound cannot be guaranteed. Multiple sprays are required to finally achieve the recovery of the burn wound, prolonging the treatment process, and the entire spray kit used is still mainly imported equipment. The price of a table is between 10,000 and 20,000, and the cost of treatment is also greatly increased accordingly.

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