Soilless culture method of Oudemansiella

A technology for soilless cultivation and oeder mushrooms, which is applied in the fields of botanical equipment and methods, horticulture, and application, can solve the problems of cultivated mushroom pollution, excessive heavy metals, etc., and achieves the effect of simple operation.

Active Publication Date: 2014-03-26
SHANGHAI ACAD OF AGRI SCI
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional cultivation of Oddya mushrooms has been widely criticized for excessive heavy metals caused by covering soil with mushrooms
Studies have shown that many mushrooms have the ability to enrich...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Soilless culture method of Oudemansiella
  • Soilless culture method of Oudemansiella
  • Soilless culture method of Oudemansiella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Strain acquisition and cultivation

[0036] The collected wild Oudemansiella submucida (collected from Zhangjiajie, Hunan) fruiting body tissue pieces were separated into PDA test tube medium, placed in a room at 18°C, and cultured without light for about 10 days, the mycelia covered the test tube , and transfer tubes for purification to obtain pure mycelia, and store them in a refrigerator at 4°C for later use;

[0037] 2. Preparation of special medium

[0038] 1) Formula and preparation of mother seed culture material: agar 20g / L, peptone 2g, fresh potato 200g (boiling liquid), citric acid 0.1g, glucose 10g, KH 2 PO 4 0.5g.VB l 10mg, CaCL 2 2H 2 O0.05g, MgSO 4 ·7H 2 0.15g, add distilled water to 1000rnL; first cook the cut potatoes with water, then filter the potatoes in the solution, then add agar to melt, add the remaining medicine after melting, and adjust the solution to 1L, pour it into a test tube to extinguish bacteria spare. The culture conditions ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a soilless culture method of Oudemansiella. The method includes the steps that firstly, sporocarps of wild Oudemansiella submucida are subjected to tissue separation and are then inoculated into a sterilized PDA test tube culture medium to obtain an Oudemansiella pure strain; secondly, the Oudemansiella pure strain obtained in the first step is inoculated into a mother strain culture material to be cultured to obtain mother strain hyphae; thirdly, the mother strain hyphae are inoculated into a strain bag of a primary strain culture material to obtain a primary strain; fourthly, the primary strain is inoculated into a strain bag of a secondary strain culture material to be cultured to obtain a secondary strain; fifthly, the secondary strain is inoculated into a strain bag of a cultivar culture material, and strain culture is carried out in a shading mode in a culture house at the temperature of 23 DEG C till hyphae grow on the whole strain bag; sixthly, fruiting and management are carried out. Compared with a conventional Oudemansiella earthing culture method, the soilless culture method of the Oudemansiella has the advantages that earthing is not needed in the fruiting process, the fruiting process is free of pollution, factory production and mass production can be achieved, and the method has uniqueness in Oudemansiella culture in China.

Description

technical field [0001] The invention belongs to the field of cultivation of ordida mushrooms, and in particular relates to a soilless cultivation method of ordida mushrooms. Background technique [0002] The cultivation and production of Oudemansiella in my country has changed from a relatively single species in the 20th century to more than 10 cultivars reported now, realizing the diversification of Oudemansiella cultivars and rewriting the pattern of single cultivars of Oudemansiella in my country. Ordinary mushrooms are famous for their crisp, delicate, slimy and soft taste, and Ordinary mushrooms have high medicinal value, and most of Ordinary mushroom species have anti-tumor, anti-oxidation and weight-loss effects, and their products must be will become more and more popular. [0003] The traditional cultivation of Oddya mushrooms has been widely criticized for excessive heavy metals caused by covering soil with mushrooms. Studies have shown that many mushrooms have the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G1/04
Inventor 李传华黄建春谭琦尚晓冬曹晖张劲松鲍大鹏沈学香
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap