Soilless culture method of Oudemansiella
A technology for soilless cultivation and oeder mushrooms, which is applied in the fields of botanical equipment and methods, horticulture, and application, can solve the problems of cultivated mushroom pollution, excessive heavy metals, etc., and achieves the effect of simple operation.
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[0035] 1. Strain acquisition and cultivation
[0036] The collected wild Oudemansiella submucida (collected from Zhangjiajie, Hunan) fruiting body tissue pieces were separated into PDA test tube medium, placed in a room at 18°C, and cultured without light for about 10 days, the mycelia covered the test tube , and transfer tubes for purification to obtain pure mycelia, and store them in a refrigerator at 4°C for later use;
[0037] 2. Preparation of special medium
[0038] 1) Formula and preparation of mother seed culture material: agar 20g / L, peptone 2g, fresh potato 200g (boiling liquid), citric acid 0.1g, glucose 10g, KH 2 PO 4 0.5g.VB l 10mg, CaCL 2 2H 2 O0.05g, MgSO 4 ·7H 2 0.15g, add distilled water to 1000rnL; first cook the cut potatoes with water, then filter the potatoes in the solution, then add agar to melt, add the remaining medicine after melting, and adjust the solution to 1L, pour it into a test tube to extinguish bacteria spare. The culture conditions ...
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