Tissue culture rapid propagation method for jonquil

A tissue culture fast propagation and longevity technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate, immature tissue culture fast propagation technology, etc., and achieve high reproduction coefficient and low cost. , the effect of short cycle

Inactive Publication Date: 2014-03-26
DASHUN INT FLOWER
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AI Technical Summary

Problems solved by technology

The cultivation of longevity flower in China is not long, the tissue culture

Method used

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Embodiment Construction

[0015] In order to understand the present invention, the present invention will be further described below through specific examples.

[0016] The preparation of the culture medium, including the components of the basic medium and the medium of each stage of tissue culture: the basic medium is: MS, the sucrose concentration is 8%, the agar component is 0.7%, and the pH value is 5.6-5.8; the callus is induced Medium: MS+6-BA1.0mg / L+NAA0.2mg / L, MS+6-BA1.0mg / L+NAA0.1mg / L; value-proliferating medium: MS+6-BA1.0mg / L+ NAA0.1mg / L; rooting medium: MS+NAA0.2mg / L;

[0017] Cultivation of virus-free tissue culture seedlings: selection and sterilization of explants: take the leaves of Changshouhua as explants, wash them with 75% alcohol for half a minute, and then disinfect them with 0.1% HgCl for 10 minutes;

[0018] Transplantation of tissue culture seedlings.

[0019] The present invention is not limited to the above-mentioned best implementation mode, and any other product identical...

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Abstract

The invention belongs to the technical field of jonquil tissue culture, and especially relates to a tissue culture rapid propagation method for jonquil. The method comprises the following steps: preparing mediums, wherein the mediums comprise a basic medium and mediums in all tissue culture stages, the compositions of the basic medium comprise MS, cane sugar with a concentration of 8% and 0.7% of agar, the pH value of the basic medium is 5.6-5.8, the compositions of an induction callus medium comprise MS + 1.0 mg/L of 6-BA + 0.2 mg/L of NAA or MS + 1.0 mg/L of 6-BA + 0.1 mg/L of NAA, the compositions of a propagation medium comprise MS + 1.0 mg/L of 6-BA + 0.1 mg/L of NAA, and the compositions of a rooting medium comprise MS + 0.2 mg/L of NAA; culturing virus-free tissue culture seedlings; selecting explants and disinfecting, specifically choosing jonquil leaves as explants, washing with alcohol with a concentration of 75% for half a minute, and then performing disinfection processing for 10 min by using HgCl with a concentration of 0.1%; and transplanting the tissue culture seedlings. The method is high in propagation coefficient, short in period and low in cost.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of longevity flower, in particular to a method for tissue culture and rapid propagation of longevity flower. Background technique [0002] Longevity flower, also known as rhodiola, is a perennial fleshy herb belonging to the sedum family and Chilancai. It likes an environment with temperature, sufficient sunlight, and good ventilation. It is resistant to drought and requires loose, fertile, and well-drained soil. The leaves of Kalanchoe are dense and emerald green, and they bloom near Christmas, clustered in clusters, with rich colors, suitable for indoor potted plants. [0003] From the end of the 1970s, began to use the stem tips, leaves, stems, flowers, etc. of Changshouhua as explants for tissue culture and rapid propagation. The cultivation of longevity flower in China is not long, and the tissue culture rapid propagation technology is immature, and the survival rate is not high. Co...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨铁顺赵亮柯盛发刘青吴建平闵宇边佩璐
Owner DASHUN INT FLOWER
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