STED (stimulated emission depletion) super-resolution microscope based on tunable laser

Abstract

The invention relates to the technical field of microscopes and provides an STED (stimulated emission depletion) super-resolution microscope based on tunable laser. The STED super-resolution microscope comprises an exciting light unit, a loss light unit, a first dichroic mirror and a scanning imaging unit, wherein the exciting light unit comprises a first tunable laser source; the loss light unit comprises a second tunable laser source, a half slide, a vortex phase plate and a quarter-wave plate; the scanning imaging unit comprises a second dichroic mirror, a scanning galvanometer, a scanning lens, a cylindrical mirror, an objective lens, a pinhole lens and a photomultiplier. According to the STED super-resolution microscope, the tunable laser sources are adopted as an exciting light source and a loss light source respectively; as the wavelengths of exciting light and loss light can be adjusted according to the needs, the proper wavelengths of the exciting light and the loss light can be found for a given fluorescent dye, and the application range of the STED super-resolution microscope is expanded.

Description

technical field [0001] The invention relates to the field of microscope technology, in particular to a STED super-resolution microscope based on tunable laser. Background technique [0002] Super-resolution optical microscopy has revolutionized research in the fields of biomedicine and materials science, and its research progress has had a profound impact in many scientific fields. There are many types of super-resolution optical microscopy due to different implementation methods: Stimulated Emission Depletion (STED) microscopy is an optical super-resolution microscopy based on laser confocal microscopy Compared with other types of super-resolution microscopy, its imaging speed is relatively fast, and it can image living cells. It is widely used in biomedical research. It can detect finer structures, and also provides a new method for research in the field of materials science. [0003] Two kinds of laser illumination are needed in the STED microscopic imaging system. One ...

AI Technical Summary

Problems solved by technology

Most of the current STED microscopic imaging systems use fixed-wavelength excitation light or depleted light, so the number of suitable fluorescent dyes is very limited, and the biological tissues that can be observed are also very limited; the use of two-color or multi-color STED can be used simultaneously. Excitation of two or more fluorescent dyes expands the scope of application of the system, but even if two-color or multi-color STED is used, the scope of application of the system is only limited. In actual biological experiments, fluorescent dyes vary widely, and a few limited The wavelength of excitation light or the wavelength of loss light cannot meet the requirements

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