A detection method for the determination of effective components of Epimedium in shredded cigarette tobacco and its mainstream smoke
A technology for mainstream smoke and active ingredients of cigarettes, applied in measuring devices, material separation, and analysis of materials, can solve the problems of inaccurate quantification, low sensitivity, and inability to quantify, and achieve high analysis and detection sensitivity, good repeatability, and improved Effects on Cigarette Product Quality
Active Publication Date: 2017-01-04
CHINA TOBACCO GUANGXI IND
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Problems solved by technology
Liquid chromatography can realize the determination of the content of the two active ingredients, but its sensitivity is low and its anti-interference ability is weak. When analyzing low-content samples, it is prone to inaccurate or impossible quantification
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[0036] Materials and Methods
[0037] 1.1 Experimental Instruments
[0038] Finnigan TSQ Quantum Discovery Max liquid chromatography-mass spectrometry (Thermo Finnigan, USA), equipped with electrospray (ESI) ion source detector; Sartorius BP110s electronic balance; RM200 smoking machine (BORGWALDT, Germany).
[0039] 1.2 Standards and samples
[0040] Icariin and Epimedin C were purchased from Tianjin Mark Biotechnology Co., Ltd.; the control cigarettes and sample cigarettes made from blank shredded tobacco of a certain brand of cigarette and the sample shredded tobacco added with Epimedium compound natural plant extract; acetonitrile was Chromatographically pure (Merck); water is high-purity water; other reagents are analytically pure.
[0041]Smoke filter: Take the selected control cigarettes and sample cigarettes, equilibrate at 22°C±1°C and RH60%±2% for 48 hours, smoke the cigarette samples according to GB / T19609 with an RM200 smoking machine, and capture them with a φ92...
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Abstract
The invention relates to a detection method for measuring effective constituents of epimedium in cigarette tobacco shreds and main stream smoke of cigarettes. According to the detection method, a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS) is utilized for detecting the content of the effective constituents, namely icariin and epimidin c, in the cigarette tobacco shreds and the main stream smoke of the cigarettes. The detection method sequentially comprises the following steps: (1) selecting and balancing the cigarettes; (2) preparing a reference substance solution; (3) preparing a cigarette tobacco shred sample solution; (4) preparing a sample solution in the main stream smoke of the cigarettes; and (5) carrying out HPLC-ESI-MS analysis on a to-be-analyzed sample. According to the detection method, the effective constituents of the epimedium in the cigarette tobacco shreds and the main stream smoke of the cigarettes can be effectively and completely extracted, and the content of the effective constituents of the epimedium in the cigarette tobacco shreds and the main stream smoke of the cigarettes can be accurately measured. The method is good in repeatability and high in analyzing and detection sensitivities.
Description
technical field [0001] The invention relates to a method for detecting the content of effective components of epimedium, in particular to a method for determining the content of effective components of epimedium in shredded cigarette tobacco and its mainstream smoke, and belongs to the technical field of tobacco detection. Background technique [0002] Epimedium is Epimedium brevicornum Maxim., Epimedium sagittatum (Sieb.et Zucc.) Maxim., Epimedium pubescens Maxim., or Epimedium koreanum The dry aerial part of Nakai has the effects of tonifying kidney yang, strengthening muscles and bones, and dispelling wind-dampness. For impotence and nocturnal emission, flaccidity of muscles and bones, rheumatic arthralgia, numbness and contracture. [0003] With the continuous advancement of the global tobacco control campaign, the public is increasingly concerned about smoking and health issues. It is believed that adding beneficial ingredients of natural plants to cigarettes can reduc...
Claims
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IPC IPC(8): G01N30/88
Inventor 吴彦范忠孟冬玲陈志燕田兆福李小兰周芸王萍娟黄东业白家峰
Owner CHINA TOBACCO GUANGXI IND