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Detection method for selectively recognizing Fe3+ capacity

A detection method and technology with capabilities, applied in the field of biometric identification, can solve the problems of poor water solubility of probes and limited sensitivity, and achieve the effects of simple and easy detection methods, high detection sensitivity, and small detection interference.

Inactive Publication Date: 2014-04-09
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Fe 3+ Important for living organisms, but for cellular Fe 3+ There are fewer probes, and due to the poor water solubility of the existing reported probes, this feature limits the measurement of Fe in cells. 3+ Increased sensitivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In order to detect the probe Fe in biological cells 3+ For the detection performance, we selected U251 glioma cells. We put U251 glioma cells into the well with a concentration of 2x10 -5 mol / L probe in tris-HCl standard buffer (pH=7.4) was incubated for 30 minutes, and then observed under a fluorescent microscope, it was obvious that the cells were stained by the probe. Then use a concentration of 2x10 -5 mol / LFe 3+ The stained cells were washed for 10 minutes, and it was observed that the fluorescence emitted by the stained cells was greatly reduced. Thereby prove, this fluorescent probe has the low concentration Fe that can be applied to organism after adopting the method of the present invention 3+ Fluorescence detection capability.

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Abstract

The invention discloses a detection method for selectively recognizing the Fe3+ capacity. The method comprises the following steps of respectively preparing a standard solution of acetonitrile with the concentration of 2*10<-5>mol / L of Ag+, Ca2+, Cd2+, Co2+, Cr3+, Cu2+, Fe3+, Hg+, Hg2+, K+, Mg2+, Mn2+, Na+, Ni2+, Pb2+ and Zn2+, and arranging 1ml of the standard solution into a cuvette; adding a probe solution into each cuvette, standing the cuvette, wherein the cuvette with Fe3+ is rapidly changed to be colorless. By adopting the method, not only can the F3+ be recognized in an organic solvent, but also the Fe3+ can be sensitively recognized in the buffering aqueous solution, the CN- with high toxicity can be recognized on the basis, and the fluorescent probe after being processed by utilizing the method can be primarily used for fluorescently recognizing Fe3+ in a cell body.

Description

technical field [0001] The invention relates to the field of biometric identification, in particular to a method for selectively identifying Fe 3+ ability to test methods. Background technique [0002] Iron is one of the most important metals in biological systems, especially ferric iron, which is the basic element of protein, cytochrome and various enzymes, and plays a vital role in the metabolism of cells. Human Fe 3+ Imbalances can cause metabolic disorder leading to anemia, liver damage, kidney failure and other diseases. Although Fe 3+ Important for living organisms, but for cellular Fe 3+ There are fewer probes, and due to the poor water solubility of the existing reported probes, this feature limits the measurement of Fe in cells. 3+ Increased sensitivity. Meanwhile, the cyanide anion has attracted increasing attention due to the high toxicity of cyanide. Cyanide ions can bind the heme cofactor and inhibit the process of cellular respiration in mammals. Most o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/52
Inventor 周为群
Owner SUZHOU UNIV
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