Three-dimensional cell-mechanical-gradient loading platform

A gradient loading, three-dimensional cell technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, stress-stimulated microbial growth methods, etc. , uneven extracellular matrix, etc., to achieve the effects of adjustable mechanical gradient, strong flexibility, and simple preparation

Active Publication Date: 2014-04-23
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The response of cells in two-dimensional culture and in vivo environment to mechanical stimuli is very different, and the research results cannot reflect the real situation of cells growing in three-dimensional state in vivo
However, these instruments can only achieve uniform mechanical stimulation, and in vivo tissues are often in a heterogeneous extracellular matrix
Considering that the existing in vitro methods for studying mechanical stimulation of cells and tissues have many limitations and deficiencies, such as complex operation, high cost, the need to use precision instruments, uniform and single mechanical stimulation, and low throughput, it is urgent to establish a simpler and more effective, Reliable new mechanical loading method

Method used

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  • Three-dimensional cell-mechanical-gradient loading platform
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  • Three-dimensional cell-mechanical-gradient loading platform

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] refer to figure 1 , using a computer to design two cantilever beams 7 in a rectangular shape (length 25mm, width 2.5mm, thickness 5mm), the distance between the two cantilever beams 7 is a three-dimensional cell gradient mechanical loading mold configuration, the mechanical loading device is made of organic material PMMA Cut to obtain a rectangular cantilever beam mold, and use a laser cutting machine to punch through holes consistent with the diameter of the threaded column at the ends of the two PMMA support cantilever beams 7, and the diameter of the threaded column 5 is 1.5mm. A three-dimensional tissue module 6 wrapped in cells is sandwiched between the two cantilever beams 7, and the contact parts of the three-dimensional tissue module 6 and the cantilever beams 7 are bonded with 502 glue. Hole, diameter 1.5mm, threaded column 5 passes through the through hole of two cantilever beams 7, the threaded column 5 on both sides of the through hole of one cantilever beam...

Embodiment 2

[0030] refer to Figure 4 , a three-dimensional cell mechanical gradient dynamic loading platform, including a mold composed of two cantilever beams 7, a three-dimensional tissue module 6 wrapped in cells is sandwiched between the two cantilever beams 7, and the contact position between the three-dimensional tissue module 6 and the cantilever beam 7 is used 502 glue connection, the ends of the two cantilever beams 7 are respectively connected to the control clips 8 provided on the one-dimensional mobile platform 9, and the two control clips 8 are respectively connected to the first motor 10 and the second motor 11. The computer can control the motor 10 and the motor 11 to move on a mobile platform, and the displacement is transmitted to the two cantilever beams 7 through the two control clamps 8 to generate a strain gradient.

Embodiment 3

[0032] refer to Figure 5, a high-throughput three-dimensional cell mechanical gradient static loading platform, a mold composed of two cantilever beam arrays with 7 arrays, a three-dimensional tissue module array wrapped in cells is sandwiched between the cantilever beam arrays, and the contact part between the three-dimensional tissue module array and the cantilever beam array Connected by 502 glue, the upper and lower ends of one cantilever beam array of the two cantilever beam arrays are respectively provided with a first plate 12 and the second plate 13, and the upper and lower ends of the other cantilever beam array are respectively provided with a third plate 14 and a fourth plate. Flat plate 15, a through hole is provided in the middle of the flat plate, a first nut 1 and a second nut 2 are provided on the threaded columns 5 on both sides of the through hole of the first flat plate 12 and the second flat plate 13 clamping a cantilever beam 7 array, A third nut 3 and a ...

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Abstract

A three-dimensional cell-mechanical-gradient loading platform comprises a mold consisting of two cantilever beams or two cantilever beam arrays, a three-dimensional tissue module or three-dimensional tissue module array for wrapping cells is clamped between the two cantilever beams or the two cantilever beam arrays, the three-dimensional tissue module and the cantilever beams are in connection by glue 502 at the contact parts; the end of each cantilever beam is provided with a through hole, and a screw-thread pillar penetrates the through holes of the two cantilever beams; the screw-thread pillar is provided with a first nut and a second nut respectively at two ends of the through hole of one cantilever beam, and the screw-thread pillar is provided with a third nut and a forth nut respectively at two ends of the through hole of the other cantilever beam; or, the ends of the two cantilever beams are respectively connected with two control clamps arranged on a one-dimensional mobile platform, and the clamps are connected with motors; or, the ends of the multiple cantilever beam arrays are fixed by upper and lower layers of flat plates, threaded columns and nuts. The three-dimensional cell mechanical gradient loading platform has the advantages of being low in cost, simple in preparation, convenient to use, controllable in dimension, adjustable in mechanical gradient, high in flux and the like.

Description

technical field [0001] The invention relates to the technical field of tissue mechanics stimulation research, in particular to a three-dimensional cell mechanics gradient loading platform. Background technique [0002] In the body, cells are in the dynamic mechanical environment provided by the body and are subjected to various mechanical stimuli from the cellular microenvironment, in which stress (strain) has a significant impact on the structure, shape and function of the tissue. However, when culturing tissues in vitro, traditional cell culture methods and culture conditions do not include these important mechanical stimuli, and it is difficult to meet the growth requirements of three-dimensional tissues and organs, causing them to lose their normal shape and function. Appropriate stress (strain) stimulation is conducive to the construction of functional three-dimensional biological tissues in vitro. Studies have shown that mechanical stress (strain) has become an import...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M35/04
Inventor 王琳王森豪徐峰卢天健
Owner XI AN JIAOTONG UNIV
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