Three-dimensional cell-mechanical-gradient loading platform

Abstract

A three-dimensional cell-mechanical-gradient loading platform comprises a mold consisting of two cantilever beams or two cantilever beam arrays, a three-dimensional tissue module or three-dimensional tissue module array for wrapping cells is clamped between the two cantilever beams or the two cantilever beam arrays, the three-dimensional tissue module and the cantilever beams are in connection by glue 502 at the contact parts; the end of each cantilever beam is provided with a through hole, and a screw-thread pillar penetrates the through holes of the two cantilever beams; the screw-thread pillar is provided with a first nut and a second nut respectively at two ends of the through hole of one cantilever beam, and the screw-thread pillar is provided with a third nut and a forth nut respectively at two ends of the through hole of the other cantilever beam; or, the ends of the two cantilever beams are respectively connected with two control clamps arranged on a one-dimensional mobile platform, and the clamps are connected with motors; or, the ends of the multiple cantilever beam arrays are fixed by upper and lower layers of flat plates, threaded columns and nuts. The three-dimensional cell mechanical gradient loading platform has the advantages of being low in cost, simple in preparation, convenient to use, controllable in dimension, adjustable in mechanical gradient, high in flux and the like.

Description

technical field [0001] The invention relates to the technical field of tissue mechanics stimulation research, in particular to a three-dimensional cell mechanics gradient loading platform. Background technique [0002] In the body, cells are in the dynamic mechanical environment provided by the body and are subjected to various mechanical stimuli from the cellular microenvironment, in which stress (strain) has a significant impact on the structure, shape and function of the tissue. However, when culturing tissues in vitro, traditional cell culture methods and culture conditions do not include these important mechanical stimuli, and it is difficult to meet the growth requirements of three-dimensional tissues and organs, causing them to lose their normal shape and function. Appropriate stress (strain) stimulation is conducive to the construction of functional three-dimensional biological tissues in vitro. Studies have shown that mechanical stress (strain) has become an import...

AI Technical Summary

Problems solved by technology

The response of cells in two-dimensional culture and in vivo environment to mechanical stimuli is very different, and the research results cannot reflect the real situation of cells growing in three-dimensional state in vivo

However, these instruments can only achieve uniform mechanical stimulation, and in vivo tissues are often in a heterogeneous extracellular matrix

Considering that the existing in vitro methods for studying mechanical stimulation of cells and tissues have many limitations and deficiencies, such as complex operation, high cost, the need to use precision instruments, uniform and single mechanical stimulation, and low throughput, it is urgent to establish a simpler and more effective, Reliable new mechanical loading method

Images