Microscopy method and device for controlled high-speed chromatography phase position

Abstract

The invention discloses a microscopy device for a controlled high-speed chromatography phase position. The microscopy device comprises a two-dimension scanning system, a first 4f system, a sample, a second 4f system, a piezoelectric ceramic control reflecting mirror, an optical path difference, a second polarization splitting prism, a polarizing film and a CCD (charge coupled device), wherein the two-dimension scanning system, the first 4f system, the sample and the second 4f system are successively arranged along a sample light path; the piezoelectric ceramic control reflecting mirror is arranged on a reference light path, and the optical path difference is used for changing the sample light path and the reference light path; the second polarization splitting prism is used for enabling the sample light and the reference light to become a bundle; the polarizing film and the CCD are successively arranged along an emergent light path of the polarization splitting prism. The invention also discloses a microscopy method of the controlled high-speed chromatography phase position. The microscopy device provided by the invention has the advantages that the system stability is high, the controllability is strong, the economy is good, the complicated angle scanning is realized, and the speed of a chromatography phase position microscopy system is increased by more than 4 times.

Description

technical field [0001] The invention belongs to the field of optical super-resolution microscopy, in particular to a microscopic method and device for controlling high-speed tomographic phase. Background technique [0002] Different cells in biological tissues have different refractive indices and undertake different organizational tasks; the nucleus, cytoplasm and various organelles in the same cell have different refractive indices. When the biological life activities change, the corresponding tissue The distribution of the refractive index of the cells in the cell will also change, such as the increase of the refractive index of cancerous cells, the change of the refractive index distribution of red blood cells infected by Plasmodium, etc. an important subject. [0003] The observation of the refractive index of cells is different from the observation of fluorescent samples and stained samples. The cells are almost in a transparent state, and the modulation of the refrac...

AI Technical Summary

Problems solved by technology

The use of acousto-optic modulators limits the data acquisition frequency of the entire system, and the control of the entire system becomes single. At the same time, the data acquisition speed of 10 frames per second is still not enough for observing rapidly changing biological activity cells, so a stable, Controllable, high-speed tomographic phase microscopy is a meaningful breakthrough in micro-optical imaging and life sciences

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