Detachable glass slide incubator

Abstract

The invention discloses a detachable glass slide incubator. The detachable glass slide incubator comprises a stainless steel incubation frame, a silica gel cushion layer, and elastic clampers which clamps the stainless steel incubation frame and the silica gel cushion layer on a glass slide, wherein the elastic clampers clamp the incubation frame and the glass slide through clamping openings of the elastic clampers; the incubation frame and the glass slide are clamped together to form a small incubation chamber through clamping parts of the elastic clampers. The detachable glass slide incubator is reasonable in structure; when immunohistochemical staining of a tissue is performed, a non-leakage incubation small chamber is formed by tightly pressing the incubation frame on the glass slide to which the tissue sections adhere by the elastic clampers repeatedly; when anti-body incubation is performed, only a trace amount of anti-body incubation liquid can ensure that all the tissue sections adhered to the glass slide are completely soaked in the anti-body incubation liquid in the process of incubating, and thus the experimental cost for scientific research is saved; other experimental steps can also be prevented from being influenced by the incubation frame by detaching the incubation frame.

Description

technical field [0001] The invention relates to a detachable glass slide incubator. Background technique [0002] In life science research, people often need to use immunohistochemical techniques with strong specificity and high sensitivity to locate and quantify specific substances in tissue cells. The basic principle of immunohistochemical technology is to first use the known immunological antibody (primary antibody) to bind to the antigen in the observed tissue. Since the antigen-antibody reaction on tissues and cells is generally invisible, it is necessary to use a labeling method to bind a certain marker (such as an enzyme, fluorescein) to the primary antibody, and then use histochemical methods to display the marker. Or observe fluorescence from fluorescein under a fluorescence microscope, confocal laser microscope, or super-resolution microscope. It is possible to visualize certain substances in the tissue of the research object. When implementing the above-mention...

AI Technical Summary

Problems solved by technology

However, although this method is an improvement over the method of directly adding the antibody incubation solution to the slide-attached tissue section, using this method, due to the poor ability of the oily frame to prevent the antibody incubation solution from spilling, it cannot be guaranteed in the future. During the entire antibody incubation process, the tissue sections are always soaked in the antibody incubation solution

Moreover, during the antibody incubation, washing, dehydration, and transparent processes, these oily materials will fall off and lose the effect of preventing the antibody incubation solution from overflowing during the antibody incubation process; and the oily materials will fall off and adhere to the tissue section, affecting the tissue section. Observed situation; when the oily material is also dissolved into the relevant experimental reagents during the tissue section processing, the reuse of the experimental reagents and the processing effect of the tissue sections are affected by the contamination of the experimental reagents

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